PMID- 22336526
OWN - NLM
STAT- MEDLINE
DCOM- 20121012
LR  - 20120619
IS  - 1879-0380 (Electronic)
IS  - 0959-437X (Linking)
VI  - 22
IP  - 3
DP  - 2012 Jun
TI  - The use of new technologies in the detection of balanced translocations in 
      hematologic disorders.
PG  - 264-71
LID - 10.1016/j.gde.2012.01.005 [doi]
AB  - The cytogenetic evaluation of hematologic disease can confirm a diagnosis, 
      determine treatment options, and provide prognostic information to the patient. 
      Among the potential cytogenetic aberrations that can be identified are certain 
      balanced translocations with recurrent breakpoints that provide disease 
      classification and define the sites of disease-causing or disease-promoting 
      genes. In this review, we discuss the importance of balanced translocation 
      identification, the methods traditionally used to identify balanced 
      translocations in the cytogenetics laboratory, and the application of new 
      methodologies such as next generation (NextGen) sequencing and array-based 
      translocation identification through a linear amplification application. These 
      new technologies have the potential to identify all currently known 
      diagnostically and prognostically important rearrangements as well as novel 
      alterations that may provide new therapeutic targets to enhance treatment of 
      hematologic disease.
CI  - Copyright (c) 2012 Elsevier Ltd. All rights reserved.
FAU - Shaffer, Lisa G
AU  - Shaffer LG
AD  - Signature Genomic Laboratories, PerkinElmer, Inc., Spokane, WA, USA. 
      lisa.shaffer@perkinelmer.com
FAU - Schultz, Roger A
AU  - Schultz RA
FAU - Ballif, Blake C
AU  - Ballif BC
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Review
DEP - 20120213
PL  - England
TA  - Curr Opin Genet Dev
JT  - Current opinion in genetics & development
JID - 9111375
RN  - 0 (DNA, Neoplasm)
SB  - IM
MH  - Chromosomes, Human/genetics
MH  - Comparative Genomic Hybridization/methods
MH  - Cytogenetics/*methods
MH  - DNA, Neoplasm/*analysis/genetics
MH  - Gene Rearrangement
MH  - Genes, Neoplasm
MH  - Genetic Testing/*methods
MH  - Genome, Human
MH  - Hematologic Neoplasms/diagnosis/*genetics
MH  - Humans
MH  - Leukemia/diagnosis/genetics
MH  - Lymphoma/diagnosis/genetics
MH  - *Translocation, Genetic
EDAT- 2012/02/18 06:00
MHDA- 2012/10/13 06:00
CRDT- 2012/02/17 06:00
PHST- 2011/12/22 00:00 [received]
PHST- 2012/01/11 00:00 [revised]
PHST- 2012/01/12 00:00 [accepted]
PHST- 2012/02/17 06:00 [entrez]
PHST- 2012/02/18 06:00 [pubmed]
PHST- 2012/10/13 06:00 [medline]
AID - S0959-437X(12)00006-8 [pii]
AID - 10.1016/j.gde.2012.01.005 [doi]
PST - ppublish
SO  - Curr Opin Genet Dev. 2012 Jun;22(3):264-71. doi: 10.1016/j.gde.2012.01.005. Epub 
      2012 Feb 13.