PMID- 22338550
OWN - NLM
STAT- MEDLINE
DCOM- 20120312
LR  - 20131121
IS  - 1128-3602 (Print)
IS  - 1128-3602 (Linking)
VI  - 16
IP  - 1
DP  - 2012 Jan
TI  - Comparative effects of zinc, selenium and vitamin E or their combination on 
      carbohydrate metabolizing enzymes and oxidative stress in streptozotocin 
      induced-diabetic rats.
PG  - 66-78
AB  - OBJECTIVE: It is well documented that oxidative stress is a basic mechanism 
      behind the development of diabetic state. The current study was undertaken to 
      elucidate the hypoglycemic role of zinc, selenium and vitamin E and their mixture 
      in comparison with the antidiabetic drug glibenclamide. MATERIALS AND METHODS: 
      Male Wistar rats weighing 250 +/- 50 g were made diabetic by injection with a 
      single i.p. dose of streptozotocin (STZ) (65 mg/kg b. wt). Diabetic groups were 
      simultaneously i.p. injected either with zinc chloride (ZnCI2) (5 mg/kg) or with 
      selenium and vitamin E (1.5 mg/kg as sodium selenite and vitamin E 1000 mg/kg) or 
      with zinc, selenium and vitamin E each element i.p. injected according to its 
      corresponding therapeutic dose daily for one month. Another group was orally 
      treated daily with glibenclamide drug (5 mg/kg) for one month. RESULTS: Blood and 
      tissue samples were collected at day 3 post STZ injection (from one group serum 
      glucose level significantly elevated < or = 300, p < or = 0.05) and at day 30 
      post-treatment in other groups. Liver function, nitric oxide (NO), 
      malondialdehyde (MDA) and phosphoenol pyruvate carboxykinase (PEPCK) were 
      significantly increased, while superoxide dismutase (SOD), reduced glutathione 
      (GSH), total protein, lactate dehydrogenase (LDH), pyruvate kinase (PK) and 
      hexokinase (HK) were inhibited after STZ treatment. Histological examination of 
      diabetic liver showed necrosis and degenerative changes of hepatocytes. Treatment 
      of diabetic rats with ZnCI2, selenium and vitamin E or their combination blunted 
      the increment in serum glucose induced by STZ, preserved liver architecture and 
      ameliorated all the previous mentioned biochemical parameters. CONCLUSIONS: It 
      was found that, the combined administration of zinc, selenium and vitamin E 
      exhibited a more remarkable effect than either zinc or selenium and vitamin E. 
      So, the results clearly indicate the beneficial effects of micronutrients 
      combination in controlling hyperglycemia.
FAU - Aly, H F
AU  - Aly HF
AD  - Therapeutical Chemistry Department, National Research Center, Giza, Egypt. 
      Hanan_abduallah@yahoo.com
FAU - Mantawy, M M
AU  - Mantawy MM
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PL  - Italy
TA  - Eur Rev Med Pharmacol Sci
JT  - European review for medical and pharmacological sciences
JID - 9717360
RN  - 0 (Antioxidants)
RN  - 0 (Blood Glucose)
RN  - 0 (Hypoglycemic Agents)
RN  - 1406-18-4 (Vitamin E)
RN  - H6241UJ22B (Selenium)
RN  - J41CSQ7QDS (Zinc)
RN  - SX6K58TVWC (Glyburide)
SB  - IM
MH  - Animals
MH  - Antioxidants/*pharmacology
MH  - Blood Chemical Analysis
MH  - Blood Glucose/metabolism
MH  - Carbohydrate Metabolism/*drug effects
MH  - Diabetes Mellitus, Experimental/*drug therapy/enzymology/metabolism
MH  - Glyburide/pharmacology
MH  - Hypoglycemic Agents/*pharmacology
MH  - Liver/chemistry/enzymology/pathology
MH  - Male
MH  - Oxidative Stress/*drug effects
MH  - Rats
MH  - Rats, Wistar
MH  - Selenium/*pharmacology
MH  - Vitamin E/*pharmacology
MH  - Zinc/*pharmacology
EDAT- 2012/02/18 06:00
MHDA- 2012/03/13 06:00
CRDT- 2012/02/18 06:00
PHST- 2012/02/18 06:00 [entrez]
PHST- 2012/02/18 06:00 [pubmed]
PHST- 2012/03/13 06:00 [medline]
PST - ppublish
SO  - Eur Rev Med Pharmacol Sci. 2012 Jan;16(1):66-78.