PMID- 22339907
OWN - NLM
STAT- MEDLINE
DCOM- 20120521
LR  - 20120220
IS  - 0253-2727 (Print)
IS  - 0253-2727 (Linking)
VI  - 32
IP  - 11
DP  - 2011 Nov
TI  - [Experimental study on rejection of allogeneic donor bone marrow cells in 
      sensitized recipients].
PG  - 734-8
AB  - OBJECTIVE: To establish a murine model of sensitization, and investigate the 
      effect and mechanism of sensitization on allogeneic donor bone marrow cells 
      (BMCs). METHODS: Sensitized BALB/c mice were established by transfusions of 
      allogeneic splenocytes. The donor reactive antibodies were detected by binding 
      and complement-dependent cytotoxicity assays. After irradiation, 1 x 10(7) BMCs 
      of C57BL/6 donor mice were injected into non-sensitized or sensitized BALB/c 
      recipient mice. The distribution pattern of donor BMCs in peripheral blood, 
      spleen and bone marrow of recipient mice were analyzed at different time points 
      (2 h, 12 h and 48 h) post transplantation. Hematopoietic recovery post 
      transplantation was assessed, and survival was monitored. Moreover, sera and 
      splenocytes derived from non-sensitized or sensitized recipients were incubated 
      with allogeneic BMCs in vitro, and the cytotoxic indexes were calculated in the 
      immune experiments. RESULTS: The binding and complement-dependent cytotoxicity 
      assays showed that a high level of donor reactive antibodies was presented in 
      sensitized sera. Compared with the non-sensitized recipients, the homing assay 
      showed significantly decreased distributions of allogeneic donor BMCs in 
      peripheral blood, spleen and femur of sensitized recipients. Non-sensitized 
      recipients survived long term after irradiation, while all the sensitized 
      recipients died within 12-15 days. Fourteen days post transplantation, the white 
      blood cells and BMCs of non-sensitized recipients were (3240 +/- 300) x 10(6)/L and 
      (396 +/- 27) x 10(6)/femur, respectively; while the white blood cells and BMCs of 
      sensitized recipients were (320 +/- 80) x 10(6)/L and (6 +/- 2) x 10(6)/femur, 
      respectively; the differences were statistically significant between this two 
      groups (P < 0.05). Seven days post transplantation, the percentage of donor cells 
      in bone marrow of non-sensitized and sensitized recipients was (48.07 +/- 4.70)% 
      and (0.77 +/- 0.11)%, respectively, and the differences were statistically 
      significant (P < 0.05). Furthermore, the white blood cells and BMCs following 
      transplantation decreased along with time in sensitized recipients. The immune 
      experiments of complement-dependent cytotoxicity reaction, cytotoxic T 
      lymphocytes reaction and antibody-dependent cellular cytotoxicity showed the 
      cytotoxic indexes were higher in sensitized group than the non-sensitized group. 
      CONCLUSION: A sensitized model was established by transfusions of allogeneic 
      spleen cells. Allogeneic donor BMCs were rejected in sensitized recipients, and 
      its mechanism might be through immune impairment pathways.
FAU - Xu, Lu-hong
AU  - Xu LH
AD  - Department of Pediatrics, Zhongshan University, Guangzhou, China.
FAU - Fang, Jian-pei
AU  - Fang JP
FAU - Weng, Wen-jun
AU  - Weng WJ
FAU - Xu, Hong-gui
AU  - Xu HG
FAU - Ye, Qi-xiang
AU  - Ye QX
LA  - chi
PT  - English Abstract
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Zhonghua Xue Ye Xue Za Zhi
JT  - Zhonghua xue ye xue za zhi = Zhonghua xueyexue zazhi
JID - 8212398
SB  - IM
MH  - Animals
MH  - *Bone Marrow Cells
MH  - *Bone Marrow Transplantation
MH  - *Disease Models, Animal
MH  - *Graft Rejection
MH  - Male
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Mice, Inbred C57BL
MH  - Tissue Donors
MH  - Transplantation, Homologous
EDAT- 2012/02/22 06:00
MHDA- 2012/05/23 06:00
CRDT- 2012/02/21 06:00
PHST- 2012/02/21 06:00 [entrez]
PHST- 2012/02/22 06:00 [pubmed]
PHST- 2012/05/23 06:00 [medline]
PST - ppublish
SO  - Zhonghua Xue Ye Xue Za Zhi. 2011 Nov;32(11):734-8.