PMID- 22340046
OWN - NLM
STAT- MEDLINE
DCOM- 20121126
LR  - 20221207
IS  - 0253-3766 (Print)
IS  - 0253-3766 (Linking)
VI  - 33
IP  - 9
DP  - 2011 Sep
TI  - [Correlation between EGFR gene mutation and high copy number and their 
      association with the clinicopathological features in Chinese patients with 
      non-small cell lung cancer].
PG  - 666-70
AB  - OBJECTIVE: The purpose of this study was to investigate the correlation between 
      gene mutation and gene copy number and their association with the clinical 
      profiles and pathological features in Chinese patients with non-small cell lung 
      cancer (NSCLC). METHODS: Surgical specimens of cancer tissue were collected from 
      118 NSCLC patients. Gene mutations in exon 19 and exon 21 were detected by 
      real-time PCR and gene copy number was detected by fluorescence in situ 
      hybridization (FISH). Chi-square (chi(2)) test was performed to analyze the 
      correlation between EGFR mutation and gene copy number, and explore their 
      association with clinicopathological features in the NSCLC patients. RESULTS: The 
      mutation frequency in EGFR was 41.5% (49/118). EGFR mutations occured in 50.0% 
      (48/96) of patients with adenocarinoma and 5.0% (1/20) of patients with squamous 
      cell carcinoma. EGFR gene high copy number was detected in 70.3% (83/118)of the 
      patients. The FISH-positive rate was 78.1% (75/96) in adenocarcinoma and 35.0% 
      (7/20) in squamous cell carcinoma. EGFR mutation and high copy number mainly 
      occurred in the adenocarcinoma, advanced stage, female gender, and non-smoking 
      patients. There was a significant correlation between EGFR gene mutation and gene 
      high copy number. CONCLUSIONS: EGFR gene mutation and gene high copy number are 
      more common in Chinese NSCLC patients with adenocarcinomas, advanced stage, 
      non-smokers and females. There is a significant correlation between gene mutation 
      and gene high copy number. Combined analysis of EGFR mutation and gene copy 
      number by FISH may provide a superior approach in selecting patients who may 
      benefit from anti-EGFR target therapy.
FAU - Li, Zhe
AU  - Li Z
AD  - Department of Thoracic Surgery, Sun Yat-sen University Cancer Center, Guangzhou, 
      China.
FAU - Zhang, Lan-jun
AU  - Zhang LJ
FAU - Wang, Wu-ping
AU  - Wang WP
FAU - Guo, Kang
AU  - Guo K
FAU - Shao, Jian-yong
AU  - Shao JY
FAU - Rong, Tie-hua
AU  - Rong TH
LA  - chi
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Zhonghua Zhong Liu Za Zhi
JT  - Zhonghua zhong liu za zhi [Chinese journal of oncology]
JID - 7910681
SB  - IM
MH  - Adenocarcinoma/*genetics/pathology
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Asian People/genetics
MH  - Carcinoma, Non-Small-Cell Lung/*genetics/pathology
MH  - Carcinoma, Squamous Cell/genetics/pathology
MH  - Exons
MH  - Female
MH  - *Gene Dosage
MH  - Genes, erbB-1/*genetics
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Lung Neoplasms/*genetics/pathology
MH  - Male
MH  - Middle Aged
MH  - *Mutation
MH  - Mutation Rate
MH  - Neoplasm Staging
MH  - Sex Factors
MH  - Smoking
EDAT- 2012/02/22 06:00
MHDA- 2012/12/10 06:00
CRDT- 2012/02/21 06:00
PHST- 2012/02/21 06:00 [entrez]
PHST- 2012/02/22 06:00 [pubmed]
PHST- 2012/12/10 06:00 [medline]
PST - ppublish
SO  - Zhonghua Zhong Liu Za Zhi. 2011 Sep;33(9):666-70.