PMID- 22340379
OWN - NLM
STAT- MEDLINE
DCOM- 20120614
LR  - 20171116
IS  - 2542-5641 (Electronic)
IS  - 0366-6999 (Linking)
VI  - 124
IP  - 24
DP  - 2011 Dec
TI  - Cellular sources of interleukin 16 in benign and malignant pleural effusions.
PG  - 4160-5
AB  - BACKGROUND: Interleukin 16 (IL-16) can be detected by ELISA in pleural effusion 
      (PE) and its concentration is higher than in serum. This study investigated the 
      cellular sources of IL-16 in PE. METHODS: The samples of PE were collected from 
      34 patients who were newly diagnosed having PE in the pleural cavity. We 
      performed cell culture to purify the pleural mesothelial cells (PMC), Wright 
      staining to count the purity and immunocytochemical stain to identify the 
      cultured cells. The intracellular IL-16 expression was detected by flow cytometry 
      (FCM). The different cells in PE were first separated by magnetic cell sorting 
      (MCAS) then the separated cells were cultured in RPMI1640 with 10% fetal calf 
      serum (FCS). We extracted the supernatant and detected IL-16 concentration by 
      ELISA. The IL-16 protein was detected by immunohistochemistry and double 
      immunofluorescence staining. RESULTS: The percentages of cells which secreted 
      IL-16 were: CD3(+)CD8(-) cells ((74.27 +/- 15.56)%, n = 34); CD3(+)CD8(+) cells 
      ((69.86 +/- 18.55)%, n = 34); CD19(+) cells ((45.30 +/- 18.77)%, n = 15); CD14(+) 
      cells ((16.91 +/- 16.69)%, n = 15); and PMC ((2.05 +/- 1.85)%, n = 7). The 
      concentrations of IL-16 in the supernatant from cultured cells were: CD4(+) cells 
      ((102.50 +/- 42.51) ng/L, n = 5); CD8(+) cells ((92.58 +/- 18.34) ng/L, n = 5); 
      CD19(+) cells ((79.85 +/- 5.62) ng/L, n = 5); CD14(+) cells ((58.51 +/- 25.38) ng/L, 
      n = 5); and PMC ((18.14 +/- 8.37) ng/L, n = 5). In lymphocytes, 
      monocytes/macrophages and PMC, we could observe the cells that expressed IL-16 
      protein. In paraffin-embedded sections, we also could observe by 
      immunohistochemistry the CD4(+)IL-16(+) cells, CD8(+)IL-16(+) cells, 
      CD19(+)IL-16(+) cells, and CD14(+)IL-16(+) cells. CONCLUSIONS: IL-16 in PE is 
      mainly secreted by T lymphocytes, including CD3(+)CD8(-) cells and CD3(+)CD8(+) 
      cells. CD19(+) cells and CD14(+) cells can also secrete IL-16, but the percentage 
      of PMC that can secrete IL-16 is very low.
FAU - Li, Jian-Jun
AU  - Li JJ
AD  - First Affiliated Hospital, Guangxi Medical University, Nanning, Guangxi 530021, 
      China.
FAU - Wei, Wei
AU  - Wei W
FAU - Shi, Huan-Zhong
AU  - Shi HZ
FAU - Li, Ying-Xin
AU  - Li YX
FAU - Mo, Wu-Ning
AU  - Mo WN
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Chin Med J (Engl)
JT  - Chinese medical journal
JID - 7513795
RN  - 0 (Antigens, CD19)
RN  - 0 (Interleukin-16)
RN  - 0 (Lipopolysaccharide Receptors)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Antigens, CD19/metabolism
MH  - CD4-Positive T-Lymphocytes/metabolism
MH  - CD8-Positive T-Lymphocytes/metabolism
MH  - Cells, Cultured
MH  - Centrifugation, Density Gradient
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Female
MH  - Flow Cytometry
MH  - Humans
MH  - Immunohistochemistry
MH  - Interleukin-16/*metabolism
MH  - Lipopolysaccharide Receptors/metabolism
MH  - Male
MH  - Middle Aged
MH  - Pleural Effusion, Malignant/*metabolism
MH  - T-Lymphocytes/metabolism
MH  - Young Adult
EDAT- 2012/02/22 06:00
MHDA- 2012/06/15 06:00
CRDT- 2012/02/21 06:00
PHST- 2012/02/21 06:00 [entrez]
PHST- 2012/02/22 06:00 [pubmed]
PHST- 2012/06/15 06:00 [medline]
PST - ppublish
SO  - Chin Med J (Engl). 2011 Dec;124(24):4160-5.