PMID- 22340401
OWN - NLM
STAT- MEDLINE
DCOM- 20120614
LR  - 20181201
IS  - 2542-5641 (Electronic)
IS  - 0366-6999 (Linking)
VI  - 124
IP  - 24
DP  - 2011 Dec
TI  - Increased expression of granulocyte colony-stimulating factor mediates 
      mesenchymal stem cells recruitment after vascular injury.
PG  - 4286-92
AB  - BACKGROUND: Recent studies indicate that bone marrow-derived cells may 
      significantly contribute to atherosclerosis, post-angioplasty restenosis and 
      transplantation-associated vasculopathy. The responsible bone marrow (BM) cells 
      and mechanisms regulating the mobilization of these cells are currently unclear. 
      The purpose of this study was to investigate the expression of granulocyte 
      colony-stimulating factor (G-CSF) on injured arteries and its effects on 
      mesenchymal stem cells (MSCs) differentiation into vascular smooth muscle cells 
      (VSMCs) in the process of vascular remodeling. METHODS: Balloon-mediated vascular 
      injury was established in female rats (n = 100) which received radioprotective 
      whole female BM cells by tail vein injection and male MSCs through a tibial BM 
      injection after lethal irradiation. The injured and contralateral carotid 
      arteries were harvested at 3, 7, 14 and 28 days after treatment. RESULTS: 
      Morphometric analysis indicated that intima to media area-ratio (I/M ratio) 
      significantly increased at 28 days, 0.899 +/- 0.057 (P < 0.01), compared with 
      uninjured arteries. Combining fluorescence in situ hybridization (FISH) and 
      immunohistochemical analysis showed that a significant number of the neointimal 
      cells derived from MSCs, (45.2 +/- 8.5)% at 28 days (P = 0.01), compared with (23.5 
      +/- 6.3)% at 14 days. G-CSF was induced in carotid arteries subject to balloon 
      angioplasty (fold mRNA change = 8.67 +/- 0.63 at three days, relative G-CSF protein 
      = 0.657 +/- 0.011 at three days, P < 0.01, respectively, compared with uninjured 
      arteries). G-CSF was chemotactic for MSCs but did not affect the differentiation 
      of MSCs into smooth-muscle-like cells. CONCLUSION: Increased expression of G-CSF 
      by injured arteries plays an essential role in contribution to recruitment and 
      homing of MSCs to the site of the arterial lesion.
FAU - Zhao, Yong
AU  - Zhao Y
AD  - Department of Cardiology, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, 
      Guangzhou, Guangdong 510120, China.
FAU - Liu, Ying-Xi
AU  - Liu YX
FAU - Xie, Shuang-Lun
AU  - Xie SL
FAU - Deng, Bing-Qing
AU  - Deng BQ
FAU - Wang, Jing-Feng
AU  - Wang JF
FAU - Nie, Ru-Qiong
AU  - Nie RQ
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Chin Med J (Engl)
JT  - Chinese medical journal
JID - 7513795
RN  - 143011-72-7 (Granulocyte Colony-Stimulating Factor)
SB  - IM
MH  - Angioplasty, Balloon
MH  - Animals
MH  - Blotting, Western
MH  - Carotid Arteries/surgery
MH  - Cell Differentiation
MH  - Cell Movement
MH  - Cells, Cultured
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Female
MH  - Granulocyte Colony-Stimulating Factor/*metabolism
MH  - Immunohistochemistry
MH  - In Situ Hybridization, Fluorescence
MH  - Male
MH  - Mesenchymal Stem Cells/*cytology
MH  - Myocytes, Smooth Muscle/cytology
MH  - Neointima/surgery/therapy
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Vascular System Injuries/*surgery/*therapy
EDAT- 2012/02/22 06:00
MHDA- 2012/06/15 06:00
CRDT- 2012/02/21 06:00
PHST- 2012/02/21 06:00 [entrez]
PHST- 2012/02/22 06:00 [pubmed]
PHST- 2012/06/15 06:00 [medline]
PST - ppublish
SO  - Chin Med J (Engl). 2011 Dec;124(24):4286-92.