PMID- 22340903
OWN - NLM
STAT- MEDLINE
DCOM- 20120802
LR  - 20221207
IS  - 1873-5835 (Electronic)
IS  - 0145-2126 (Linking)
VI  - 36
IP  - 7
DP  - 2012 Jul
TI  - Establishment and characterization of a new human acute myelomonocytic leukemia 
      cell line JIH-3.
PG  - 889-94
LID - 10.1016/j.leukres.2012.01.012 [doi]
AB  - Here, a new acute myelomonocytic leukemia (AMML) cell line, JIH-3, is reported, 
      and its biological characteristics are described. JIH-3 cells were maintained 
      without any cytokines for 27 months. The JIH-3 cell line showed typical 
      myelomonocytic morphological features. Additionally, it mainly expressed myeloid 
      and monocytic markers (CD13, CD14, CD11b, CD15 and CD33), although it also 
      expressed other antigens such as the markers of T and B lymphocytic lineage as 
      well as stem cell, progenitor cell, and natural killer cell-related antigens 
      (CD4, CD5, CD7, CD10, CD22, CD34, CD38, HLADR, CD16/CD56 and CD56); the 
      expression of these markers, suggested that this cell line was in the early stage 
      of myelomonocytic differentiation. Cytogenetic analysis initially showed a 
      karyotype of 46, XY, del(7) (p1?3p2?2). During the passage period, the cells with 
      this karyotype gradually decreased and were replaced by cells with a 
      45,XY,dic(4;7)(p11;p11),del(15)(q2?2) karyotype. Chromosome painting showed a 
      deletion in the short arm of chromosome 7 for del(7)(p1?3p2?2) and 
      der(4;7)(p11;p11). The latter had larger deleted segment than the former. 
      Fluorescence in situ hybridization (FISH) revealed the dicentric nature of 
      der(4;7), and Multiplex FISH (M-FISH) confirmed that der(4;7) was an unbalanced 
      translocation. A deletion involving the 7p region on dic(4;7)(p11;p11) harbors 
      many genes, including CDC2L5, C7ORF11, C7ORF10 and INHBA. Haploinsufficiency of 
      the genes on 4p, 7p and 15q caused by deletions of 4p, 7p and 15q2?2 that 
      resulted from dic(4;7)(p11;p11) and del(15)(q2?2) may play important roles in 
      leukemogenesis and in the establishment of the JIH-3 cell line. JIH-3 cells did 
      not express multidrug resistance (MDR)-related genes and apoptosis-related genes 
      such as MDR1, multidrug resistance-related protein, lung resistance protein, 
      BCL-2, Bax, GS-pi or Fax, only P21 expression was detected, which probably leads 
      the MDR indirectly through inhibition of the activities of cyclin-dependent 
      kinase (CDK). JIH-3 cells had tumorigenic capacity in nude mice. In conclusion, 
      JIH-3 is a new acute myelomonocytic leukemia cell line. It is from a 
      well-characterized background and provides a new useful tool for the study of 
      leukemia patients with a 7p deletion.
CI  - Copyright (c) 2012 Elsevier Ltd. All rights reserved.
FAU - Pan, Jinlan
AU  - Pan J
AD  - The First Affiliated Hospital of Soochow University, Jiangsu Institute of 
      Hematology, Key Laboratory of Thrombosis and Hemostasis, Suzhou, PR China.
FAU - Xue, Yongquan
AU  - Xue Y
FAU - Chen, Suning
AU  - Chen S
FAU - Qiu, Huiying
AU  - Qiu H
FAU - Wu, Chunxiao
AU  - Wu C
FAU - Jiang, Hui
AU  - Jiang H
FAU - Wang, Qian
AU  - Wang Q
FAU - Zhang, Jun
AU  - Zhang J
FAU - Bai, Suxiao
AU  - Bai S
FAU - Wu, Yafang
AU  - Wu Y
FAU - Wang, Yong
AU  - Wang Y
FAU - Shen, Juan
AU  - Shen J
LA  - eng
PT  - Case Reports
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20120214
PL  - England
TA  - Leuk Res
JT  - Leukemia research
JID - 7706787
SB  - IM
MH  - Animals
MH  - Asian People
MH  - Cell Line, Tumor
MH  - Cytogenetic Analysis
MH  - Humans
MH  - Immunophenotyping
MH  - Leukemia, Myelomonocytic, Acute/genetics/*pathology
MH  - Mice
MH  - Mice, Nude
MH  - Middle Aged
MH  - Neoplasm Transplantation/pathology
MH  - *Primary Cell Culture/methods
MH  - Transplantation, Heterologous
EDAT- 2012/02/22 06:00
MHDA- 2012/08/03 06:00
CRDT- 2012/02/21 06:00
PHST- 2011/10/26 00:00 [received]
PHST- 2011/12/31 00:00 [revised]
PHST- 2012/01/19 00:00 [accepted]
PHST- 2012/02/21 06:00 [entrez]
PHST- 2012/02/22 06:00 [pubmed]
PHST- 2012/08/03 06:00 [medline]
AID - S0145-2126(12)00031-8 [pii]
AID - 10.1016/j.leukres.2012.01.012 [doi]
PST - ppublish
SO  - Leuk Res. 2012 Jul;36(7):889-94. doi: 10.1016/j.leukres.2012.01.012. Epub 2012 
      Feb 14.