PMID- 22341322
OWN - NLM
STAT- MEDLINE
DCOM- 20120716
LR  - 20131121
IS  - 1875-7855 (Electronic)
IS  - 0079-6123 (Linking)
VI  - 196
DP  - 2012
TI  - Neural activity imaging with genetically encoded calcium indicators.
PG  - 79-94
LID - 10.1016/B978-0-444-59426-6.00005-7 [doi]
AB  - Genetically encoded calcium indicators (GECIs), together with modern microscopy, 
      allow repeated activity measurement, in real time and with cellular resolution, 
      of defined cellular populations. Recent efforts in protein engineering have 
      yielded several high-quality GECIs that facilitate new applications in 
      neuroscience. Here, we summarize recent progress in GECI design, optimization, 
      and characterization, and provide guidelines for selecting the appropriate GECI 
      for a given biological application. We focus on the unique challenges associated 
      with imaging in behaving animals.
CI  - Copyright A(c) 2012 Elsevier B.V. All rights reserved.
FAU - Tian, Lin
AU  - Tian L
AD  - Howard Hughes Medical Institute, Janelia Farm Research Campus, Ashburn, VA, USA. 
      lintian@ucdavis.edu
FAU - Akerboom, Jasper
AU  - Akerboom J
FAU - Schreiter, Eric R
AU  - Schreiter ER
FAU - Looger, Loren L
AU  - Looger LL
LA  - eng
PT  - Journal Article
PT  - Review
PL  - Netherlands
TA  - Prog Brain Res
JT  - Progress in brain research
JID - 0376441
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - Animals
MH  - Calcium/*metabolism
MH  - Calcium Signaling/*genetics
MH  - Molecular Imaging/*methods
MH  - Neurons/*metabolism
EDAT- 2012/02/22 06:00
MHDA- 2012/07/17 06:00
CRDT- 2012/02/21 06:00
PHST- 2012/02/21 06:00 [entrez]
PHST- 2012/02/22 06:00 [pubmed]
PHST- 2012/07/17 06:00 [medline]
AID - B978-0-444-59426-6.00005-7 [pii]
AID - 10.1016/B978-0-444-59426-6.00005-7 [doi]
PST - ppublish
SO  - Prog Brain Res. 2012;196:79-94. doi: 10.1016/B978-0-444-59426-6.00005-7.