PMID- 22342981
OWN - NLM
STAT- MEDLINE
DCOM- 20120507
LR  - 20120803
IS  - 1090-2104 (Electronic)
IS  - 0006-291X (Linking)
VI  - 419
IP  - 2
DP  - 2012 Mar 9
TI  - Retracted: Overexpression of alphaCP2, a translational repressor of GAP-43, inhibited 
      axon outgrowth during development in Xenopus laevis.
PG  - 262-7
LID - 10.1016/j.bbrc.2012.02.006 [doi]
AB  - The 3'-untranslated regions (3'-UTRs) of growth-associated protein 43 (GAP-43), 
      which is crucial for neural development and axonal regeneration, are highly 
      conserved among vertebrates. Previous studies in mammals have identified one 
      U-rich cis element within GAP-43 3'-UTR and several trans factors that regulate 
      its mRNA stability. However, much less is known in lower vertebrates. The Xenopus 
      GAP-43 3'-UTR, despite its high similarity with those in higher vertebrates, 
      contains unique CU-rich sequences, suggesting the existence of novel cis elements 
      and trans factors. In current study, we isolated four proteins bound to GAP-43 
      3'-UTR from juvenile frog brain using affinity purification. Mass spectrometry 
      identified Hu antigen D (HuD) and poly(C) binding protein 2 (alphaCP2) as the 
      proteins forming 48- and 44-kDa ribonucleoprotein complexes, respectively. We 
      validated the association between alphaCP2 and GAP-43 3'-UTR in vivo. After 
      confirming the post-transcriptional effects of alphaCP2 on GAP-43 expression, we 
      demonstrated that alphaCP2 directly inhibited the translation of GAP-43 gene, without 
      affecting its mRNA stability. alphaCP2 overexpression led to decreased level of 
      GAP-43 protein and significantly inhibited axonal outgrowth in primarily cultured 
      neurons. Our study therefore provided insights on novel functions of alphaCP2 in 
      vertebrate nervous system during development and new mechanisms of 
      post-transcriptional regulation for GAP-43 gene.
CI  - Copyright (c) 2012 Elsevier Inc. All rights reserved.
FAU - Wang, Jing
AU  - Wang J
AD  - Department of Radiology, Union Hospital, Tongji Medical College, Huazhong 
      University of Science and Technology, Wuhan, Hubei 430022, China.
FAU - Sun, Shenggang
AU  - Sun S
FAU - Cao, Xuebing
AU  - Cao X
FAU - Deng, Xuejun
AU  - Deng X
FAU - Zhang, Yunjian
AU  - Zhang Y
FAU - Zhu, Qing
AU  - Zhu Q
LA  - eng
PT  - Journal Article
PT  - Retracted Publication
DEP - 20120210
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (3' Untranslated Regions)
RN  - 0 (GAP-43 Protein)
RN  - 0 (Repressor Proteins)
SB  - IM
RIN - Biochem Biophys Res Commun. 2012 May 11;421(3):634. PMID: 22730563
MH  - 3' Untranslated Regions
MH  - Amino Acid Sequence
MH  - Animals
MH  - Axons/metabolism/*physiology
MH  - Brain/embryology/metabolism
MH  - Cells, Cultured
MH  - GAP-43 Protein/*genetics
MH  - *Gene Expression Regulation, Developmental
MH  - Molecular Sequence Data
MH  - Neurons/metabolism/physiology
MH  - Protein Biosynthesis/*genetics
MH  - RNA Stability
MH  - Repressor Proteins/genetics/*metabolism
MH  - Transcription, Genetic
MH  - Xenopus laevis/*embryology/genetics/metabolism
EDAT- 2012/02/22 06:00
MHDA- 2012/05/09 06:00
CRDT- 2012/02/21 06:00
PHST- 2012/01/31 00:00 [received]
PHST- 2012/02/02 00:00 [accepted]
PHST- 2012/02/21 06:00 [entrez]
PHST- 2012/02/22 06:00 [pubmed]
PHST- 2012/05/09 06:00 [medline]
AID - S0006-291X(12)00224-0 [pii]
AID - 10.1016/j.bbrc.2012.02.006 [doi]
PST - ppublish
SO  - Biochem Biophys Res Commun. 2012 Mar 9;419(2):262-7. doi: 
      10.1016/j.bbrc.2012.02.006. Epub 2012 Feb 10.