PMID- 22344928
OWN - NLM
STAT- MEDLINE
DCOM- 20120828
LR  - 20160511
IS  - 2042-6984 (Electronic)
IS  - 2042-6976 (Linking)
VI  - 2
IP  - 3
DP  - 2012 May-Jun
TI  - Increased percentage of mast cells within sinonasal mucosa of chronic 
      rhinosinusitis with nasal polyp patients independent of atopy.
PG  - 233-40
LID - 10.1002/alr.21021 [doi]
AB  - BACKGROUND: Initial attention on the pathophysiology of chronic rhinosinusitis 
      (CRS) has focused on eosinophils. Other immune cells such as mast cells (MCs) 
      have been identified and appear to be elevated in CRS with nasal polyp (NP) 
      patients. MCs are commonly linked to immunoglobulin E (IgE)-mediated inflammatory 
      changes characterized by elevated T helper 2 cytokines. Although atopy is a 
      common comorbid condition with CRS, the objective of this study was to determine 
      if elevated MCs are linked primarily to atopic status in CRS patients and to 
      understand the significance of MCs in the pathophysiology of CRS. METHODS: 
      Ethmoid sinonasal mucosa from patients undergoing endoscopic sinus surgery was 
      harvested from 3 groups: healthy control (HC), CRS without NP (CRSsNP), and CRS 
      with NP (CRSwNP) and analyzed by flow cytometry to quantify CD117(+) /CD203c(+) 
      MCs and CRTH2(+) CD4(+) T cells. Relative expression of prostaglandin D(2) 
      synthase in ethmoid mucosa was determined by quantitative real-time polymerase 
      chain reaction (PCR). RESULTS: MCs were significantly elevated in CRSwNP patients 
      as compared to CRSsNP patients and HCs. This elevation was not solely dependent 
      on the presence of IgE-mediated hypersensitivity. Relative expression of 
      prostaglandin D(2) synthase was also increased in CRSwNP patients along with an 
      associated presence of a CRTH2(+) memory CD4(+) T cell population. CONCLUSION: 
      Elevated percentages of MCs are found in the sinonasal mucosa of CRSwNP patients, 
      regardless of atopic status. Secreted by MCs, elevated prostaglandin D(2) may 
      play a role in the recruitment of CRTH2(+) cells to the inflamed mucosa of CRSwNP 
      patients.
CI  - Copyright (c) 2012 American Rhinologic Society-American Academy of Otolaryngic 
      Allergy, LLC.
FAU - Shaw, Joanne L
AU  - Shaw JL
AD  - Department of Otorhinolaryngology-Head & Neck Surgery and Texas Sinus Institute, 
      University of Texas Medical School at Houston, TX 77030, USA.
FAU - Ashoori, Faramarz
AU  - Ashoori F
FAU - Fakhri, Samer
AU  - Fakhri S
FAU - Citardi, Martin J
AU  - Citardi MJ
FAU - Luong, Amber
AU  - Luong A
LA  - eng
PT  - Journal Article
DEP - 20120216
PL  - United States
TA  - Int Forum Allergy Rhinol
JT  - International forum of allergy & rhinology
JID - 101550261
RN  - 0 (Lipocalins)
RN  - 0 (RNA, Messenger)
RN  - EC 5.3.- (Intramolecular Oxidoreductases)
RN  - EC 5.3.99.2 (prostaglandin R2 D-isomerase)
SB  - IM
MH  - CD4-Positive T-Lymphocytes/pathology
MH  - Case-Control Studies
MH  - Chronic Disease
MH  - Endoscopy
MH  - Ethmoid Sinusitis/*pathology
MH  - Humans
MH  - Hypersensitivity/pathology
MH  - Intramolecular Oxidoreductases/metabolism
MH  - Lipocalins/metabolism
MH  - Mast Cells/*pathology
MH  - Microscopy, Electron, Scanning
MH  - Nasal Mucosa/pathology
MH  - Nasal Polyps/*pathology
MH  - RNA, Messenger/metabolism
MH  - Respiratory Mucosa/pathology
MH  - Rhinitis/*pathology
EDAT- 2012/02/22 06:00
MHDA- 2012/08/29 06:00
CRDT- 2012/02/21 06:00
PHST- 2011/08/15 00:00 [received]
PHST- 2011/12/01 00:00 [revised]
PHST- 2011/12/19 00:00 [accepted]
PHST- 2012/02/21 06:00 [entrez]
PHST- 2012/02/22 06:00 [pubmed]
PHST- 2012/08/29 06:00 [medline]
AID - 10.1002/alr.21021 [doi]
PST - ppublish
SO  - Int Forum Allergy Rhinol. 2012 May-Jun;2(3):233-40. doi: 10.1002/alr.21021. Epub 
      2012 Feb 16.