PMID- 22345444
OWN - NLM
STAT- MEDLINE
DCOM- 20120618
LR  - 20211021
IS  - 1098-5514 (Electronic)
IS  - 0022-538X (Print)
IS  - 0022-538X (Linking)
VI  - 86
IP  - 9
DP  - 2012 May
TI  - Measles virus glycoprotein-pseudotyped lentiviral vectors are highly superior to 
      vesicular stomatitis virus G pseudotypes for genetic modification of 
      monocyte-derived dendritic cells.
PG  - 5192-203
LID - 10.1128/JVI.06283-11 [doi]
AB  - Dendritic cells (DCs) are potent antigen-presenting cells capable of promoting or 
      regulating innate and adaptive immune responses against non-self antigens. To 
      better understand the DC biology or to use them for immune intervention, a 
      tremendous effort has been made to improve gene transfer in these cells. 
      Lentiviral vectors (LVs) have conferred a huge advantage in that they can 
      transduce nondividing cells such as human monocyte-derived DCs (MDDCs) but 
      required high amounts of viral particles and/or accessory proteins such as Vpx or 
      Vpr to achieve sufficient transduction rates. As a consequence, these LVs have 
      been shown to cause dramatic functional modifications, such as the activation or 
      maturation of transduced MDDCs. Taking advantage of new pseudotyped LVs, i.e., 
      with envelope glycoproteins from the measles virus (MV), we demonstrate that 
      MDDCs are transduced very efficiently with these new LVs compared to the 
      classically used vesicular stomatitis virus G-pseudotyped LVs and thus allowed to 
      achieve high transduction rates at relatively low multiplicities of infection. 
      Moreover, in this experimental setting, no activation or maturation markers were 
      upregulated, while MV-LV-transduced cells remained able to mature after an 
      appropriate Toll-like receptor stimulation. We then demonstrate that our 
      MV-pseudotyped LVs use DC-SIGN, CD46, and CD150/SLAM as receptors to transduce 
      MDDCs. Altogether, our results show that MV-pseudotyped LVs provide the most 
      accurate and simple viral method for efficiently transferring genes into MDDCs 
      without affecting their activation and/or maturation status.
FAU - Humbert, J-M
AU  - Humbert JM
AD  - INSERM UMR643-Institute for Transplantation, Urology, and Nephrology, Nantes, 
      France.
FAU - Frecha, C
AU  - Frecha C
FAU - Amirache Bouafia, F
AU  - Amirache Bouafia F
FAU - N'Guyen, T H
AU  - N'Guyen TH
FAU - Boni, S
AU  - Boni S
FAU - Cosset, F-L
AU  - Cosset FL
FAU - Verhoeyen, E
AU  - Verhoeyen E
FAU - Halary, F
AU  - Halary F
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20120215
PL  - United States
TA  - J Virol
JT  - Journal of virology
JID - 0113724
RN  - 0 (Cell Adhesion Molecules)
RN  - 0 (DC-specific ICAM-3 grabbing nonintegrin)
RN  - 0 (Lectins, C-Type)
RN  - 0 (Membrane Cofactor Protein)
RN  - 0 (Receptors, Cell Surface)
RN  - 0 (Toll-Like Receptor 3)
RN  - 0 (Viral Envelope Proteins)
SB  - IM
MH  - Cell Adhesion Molecules/genetics
MH  - Cell Differentiation
MH  - Dendritic Cells/cytology/*metabolism/virology
MH  - Gene Expression
MH  - Gene Transfer Techniques
MH  - Genetic Vectors/*genetics
MH  - Humans
MH  - Lectins, C-Type/genetics
MH  - Lentivirus/*genetics
MH  - Measles virus/*genetics
MH  - Membrane Cofactor Protein/genetics
MH  - Receptors, Cell Surface/genetics
MH  - Resting Phase, Cell Cycle
MH  - Toll-Like Receptor 3/agonists
MH  - *Transduction, Genetic
MH  - Vesicular stomatitis Indiana virus/*genetics
MH  - Viral Envelope Proteins/*genetics
PMC - PMC3347358
EDAT- 2012/02/22 06:00
MHDA- 2012/06/19 06:00
PMCR- 2012/11/01
CRDT- 2012/02/21 06:00
PHST- 2012/02/21 06:00 [entrez]
PHST- 2012/02/22 06:00 [pubmed]
PHST- 2012/06/19 06:00 [medline]
PHST- 2012/11/01 00:00 [pmc-release]
AID - JVI.06283-11 [pii]
AID - 06283-11 [pii]
AID - 10.1128/JVI.06283-11 [doi]
PST - ppublish
SO  - J Virol. 2012 May;86(9):5192-203. doi: 10.1128/JVI.06283-11. Epub 2012 Feb 15.