PMID- 22348301
OWN - NLM
STAT- MEDLINE
DCOM- 20120709
LR  - 20211021
IS  - 1475-2875 (Electronic)
IS  - 1475-2875 (Linking)
VI  - 11
DP  - 2012 Feb 20
TI  - Polysaccharides from the Chinese medicinal herb Achyranthes bidentata enhance 
      anti-malarial immunity during Plasmodium yoelii 17XL infection in mice.
PG  - 49
LID - 10.1186/1475-2875-11-49 [doi]
AB  - BACKGROUND: Clinical immunity to malaria in human populations is developed after 
      repeated exposure to malaria. Regulation and balance of host immune responses may 
      lead to optimal immunity against malaria parasite infection. Polysaccharides 
      (ABPS) derived from the Chinese herb ox knee Achyranthes bidentata possess 
      immuno-modulatory functions. The aim of this study is to use the rodent malaria 
      model Plasmodium yoelii 17XL (P. y17XL) to examine whether pretreatment with ABPS 
      will modulate host immunity against malaria infection and improve the outcome of 
      the disease. METHODS: To determine whether ABPS could modulate immunity against 
      malaria, mice were pretreated with ABPS prior to blood-stage infection by P. 
      y17XL. Host survival and parasitaemia were monitored daily. The effect of 
      pretreatment on host immune responses was studied through the quantitation of 
      cytokines, dendritic cell populations, and natural regulatory T cells (Treg). 
      RESULTS: Pretreatment with ABPS prior to infection significantly extended the 
      survival time of mice after P. y17XL infection. At three and five days 
      post-infection, ABPS pretreated mice developed stronger Th1 immune responses 
      against malaria infection with the number of F4/80+CD36+ macrophages and levels 
      of IFN-gamma, TNF-alpha and nitric oxide being significantly higher than in the control 
      group. More importantly, ABPS-treated mice developed more myeloid (CD11c+CD11b+) 
      and plasmacytoid dendritic cells (CD11c+CD45R+/B220+) than control mice. ABPS 
      pretreatment also resulted in modulated expression of MHC-II, CD86, and 
      especially Toll-like receptor 9 by CD11c+ dendritic cells. In comparison, 
      pretreatment with ABPS did not alter the number of natural Treg or the production 
      of the anti-inflammatory cytokine IL-10. CONCLUSION: Pretreatment with the 
      immuno-modulatory ABPS selectively enhanced Th1 immune responses to control the 
      proliferation of malaria parasites, and prolonged the survival of mice during 
      subsequent malaria infection.
FAU - Zhu, Xiaotong
AU  - Zhu X
AD  - Department of Immunology, College of Basic Medical Sciences, China Medical 
      University, No,92, Bei'er Road, Heping District, Shenyang, Liaoning 110001, 
      China.
FAU - Pan, Yanyan
AU  - Pan Y
FAU - Zheng, Li
AU  - Zheng L
FAU - Cui, Liwang
AU  - Cui L
FAU - Cao, Yaming
AU  - Cao Y
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20120220
PL  - England
TA  - Malar J
JT  - Malaria journal
JID - 101139802
RN  - 0 (Antigens, CD)
RN  - 0 (Cytokines)
RN  - 0 (Drugs, Chinese Herbal)
RN  - 0 (Immunologic Factors)
RN  - 0 (Polysaccharides)
SB  - IM
MH  - Achyranthes/*chemistry
MH  - Animals
MH  - Antigens, CD/biosynthesis/immunology
MH  - Cytokines/biosynthesis/immunology
MH  - Dendritic Cells/drug effects/immunology/parasitology
MH  - Drugs, Chinese Herbal/isolation & purification/pharmacology
MH  - Female
MH  - Humans
MH  - Immunity, Cellular/*drug effects
MH  - Immunologic Factors/isolation & purification/*pharmacology
MH  - Macrophages/drug effects/immunology/parasitology
MH  - Malaria/drug therapy/*immunology/mortality/parasitology
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Plasmodium yoelii/*drug effects/immunology
MH  - Polysaccharides/isolation & purification/*pharmacology
MH  - Survival Rate
MH  - Th1-Th2 Balance
PMC - PMC3312874
EDAT- 2012/02/22 06:00
MHDA- 2012/07/10 06:00
PMCR- 2012/02/20
CRDT- 2012/02/22 06:00
PHST- 2011/12/14 00:00 [received]
PHST- 2012/02/20 00:00 [accepted]
PHST- 2012/02/22 06:00 [entrez]
PHST- 2012/02/22 06:00 [pubmed]
PHST- 2012/07/10 06:00 [medline]
PHST- 2012/02/20 00:00 [pmc-release]
AID - 1475-2875-11-49 [pii]
AID - 10.1186/1475-2875-11-49 [doi]
PST - epublish
SO  - Malar J. 2012 Feb 20;11:49. doi: 10.1186/1475-2875-11-49.