PMID- 22350221
OWN - NLM
STAT- MEDLINE
DCOM- 20120911
LR  - 20220325
IS  - 1573-2592 (Electronic)
IS  - 0271-9142 (Print)
IS  - 0271-9142 (Linking)
VI  - 32
IP  - 3
DP  - 2012 Jun
TI  - Modulation of mTOR effector phosphoproteins in blood basophils from allergic 
      patients.
PG  - 565-73
LID - 10.1007/s10875-012-9651-x [doi]
AB  - The mammalian target of rapamycin (mTOR) pathway contributes to various 
      immunoinflammatory processes. Yet, its potential involvement in basophil 
      responses in allergy remains unclear. In this pilot study, we quantified two key 
      mTOR effector phosphoproteins, the eukaryotic initiation factor 4E (peIF4E) and 
      S6 ribosomal protein (pS6rp), in blood basophils from nut allergy patients (NA, 
      N = 16) and healthy controls (HC, N = 13). Without stimulation in vitro, basophil 
      peIF4E levels were higher in NA than HC subjects (P = 0.014). Stimulation with 
      nut (offending) but not chicken / rice (non-offending) extract increased basophil 
      peIF4E and pS6rp levels (+32%, P = 0.018, and +98%, P = 0.0026, respectively) in 
      NA but not HC subjects, concomitant with increased surface levels of CD203c and 
      CD63, both known to reflect basophil activation. Pre-treatment with the mTOR 
      inhibitor rapamycin decreased pS6rp and CD203c responses in nut 
      extract-stimulated basophils in NA subjects. Thus, basophil responses to 
      offending allergens are associated with modulation of mTOR effector 
      phosphoproteins.
FAU - Gernez, Yael
AU  - Gernez Y
AD  - Department of Genetics, Stanford University School of Medicine, Stanford, CA 
      94305, USA.
FAU - Tirouvanziam, Rabindra
AU  - Tirouvanziam R
FAU - Reshamwala, Neha
AU  - Reshamwala N
FAU - Yu, Grace
AU  - Yu G
FAU - Weldon, Brittany C
AU  - Weldon BC
FAU - Galli, Stephen J
AU  - Galli SJ
FAU - Herzenberg, Leonore A
AU  - Herzenberg LA
FAU - Nadeau, Kari C
AU  - Nadeau KC
LA  - eng
GR  - U01 AI147462/AI/NIAID NIH HHS/United States
GR  - R01 AI023990/AI/NIAID NIH HHS/United States
GR  - AI23990/AI/NIAID NIH HHS/United States
GR  - P20 ES018173/ES/NIEHS NIH HHS/United States
GR  - AI070813/AI/NIAID NIH HHS/United States
GR  - R01 CA072074/CA/NCI NIH HHS/United States
GR  - CA72074/CA/NCI NIH HHS/United States
GR  - R37 AI023990/AI/NIAID NIH HHS/United States
GR  - R01 AI070813/AI/NIAID NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20120221
PL  - Netherlands
TA  - J Clin Immunol
JT  - Journal of clinical immunology
JID - 8102137
RN  - 0 (Allergens)
RN  - 0 (Eukaryotic Initiation Factor-4E)
RN  - 0 (Plant Extracts)
RN  - 0 (Ribosomal Protein S6)
RN  - EC 2.7.1.1 (MTOR protein, human)
RN  - EC 2.7.11.1 (TOR Serine-Threonine Kinases)
RN  - W36ZG6FT64 (Sirolimus)
SB  - IM
MH  - Adolescent
MH  - Allergens/immunology/pharmacology
MH  - Anacardium/immunology
MH  - Basophils/drug effects/*immunology
MH  - Child
MH  - Child, Preschool
MH  - Eukaryotic Initiation Factor-4E/*immunology
MH  - Female
MH  - Humans
MH  - Juglans/immunology
MH  - Male
MH  - Nut Hypersensitivity/blood/*immunology
MH  - Peanut Hypersensitivity/blood/*immunology
MH  - Phosphorylation
MH  - Plant Extracts/immunology/pharmacology
MH  - Ribosomal Protein S6/*immunology
MH  - Sirolimus/pharmacology
MH  - TOR Serine-Threonine Kinases/antagonists & inhibitors/*immunology
MH  - Young Adult
PMC - PMC4161465
MID - NIHMS606227
COIS- Competing interest statement The authors declare to hold no conflict of interest 
      with the publication of the results included in this manuscript.
EDAT- 2012/02/22 06:00
MHDA- 2012/09/12 06:00
PMCR- 2014/09/11
CRDT- 2012/02/22 06:00
PHST- 2011/08/01 00:00 [received]
PHST- 2012/01/10 00:00 [accepted]
PHST- 2012/02/22 06:00 [entrez]
PHST- 2012/02/22 06:00 [pubmed]
PHST- 2012/09/12 06:00 [medline]
PHST- 2014/09/11 00:00 [pmc-release]
AID - 10.1007/s10875-012-9651-x [doi]
PST - ppublish
SO  - J Clin Immunol. 2012 Jun;32(3):565-73. doi: 10.1007/s10875-012-9651-x. Epub 2012 
      Feb 21.