PMID- 22351283
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20121002
LR  - 20120221
IS  - 1543-1894 (Print)
IS  - 1543-1894 (Linking)
VI  - 38
DP  - 2000
TI  - Dendritic cells in old age.
PG  - 291-309
LID - 10.1385/1-59259-070-5:291 [doi]
AB  - Dendritic cells (DCs) are powerful antigen-presenting cells that have the unique 
      capacity to stimulate naive T-cells (1, 2). DCs are identified by a triad of 
      criteria: Morphologically, they exhibit pronounced cytoplasmic veils that are 
      mobile and can easily be observed under a phase-contrast microscope. These veils 
      become apparent only in the mature state. Phenotypically, they express high 
      levels of major histocompatibility class (MHC) (class I and II), adhesion (CD11c, 
      CD54, CD58), and costimulatory (CD80, CD86, CD40) molecules on their cell 
      surfaces. They also express CD1a and CD83, but lack CD14. On cytocentrifuge 
      smears stained with anti-CD68, a marker of the endocytic system that is abundant 
      in macrophages, DCs display spotlike staining whereas typical macrophages are 
      strongly positive all over the cytoplasm. When looking at forward/side scatter 
      profiles in the fluorescence-activated cell sorter (FACS), DCs show high light 
      scattering and are outside the typical lymphocyte gate. Functionally, they are 
      potent stimulators of resting T lymphocytes in the allogeneic mixed leukocyte 
      reaction. DCs derived from various tissues have been shown to undergo a complex 
      maturation process during which their morphology, phenotype, and function change. 
      DCs are derived from bone marrow progenitors and circulate in the blood as 
      immature precursors before they migrate into peripheral tissues, such as the 
      epidermis, heart, lung, liver, gut, thymus, spleen, and lymph nodes. DCs of 
      myeloid as well as of lymphoid origin have been described (3-5).
FAU - Grubeck-Loebenstein, B
AU  - Grubeck-Loebenstein B
AD  - Institute for Biomedical Aging Research of the Austrian Academy of Sciences, 
      Innsbruck, Austria.
FAU - Saurwein-Teissl, M
AU  - Saurwein-Teissl M
FAU - Romani, N
AU  - Romani N
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Methods Mol Med
JT  - Methods in molecular medicine
JID - 101123138
EDAT- 2000/01/01 00:00
MHDA- 2000/01/01 00:01
CRDT- 2012/02/22 06:00
PHST- 2012/02/22 06:00 [entrez]
PHST- 2000/01/01 00:00 [pubmed]
PHST- 2000/01/01 00:01 [medline]
AID - 10.1385/1-59259-070-5:291 [doi]
PST - ppublish
SO  - Methods Mol Med. 2000;38:291-309. doi: 10.1385/1-59259-070-5:291.