PMID- 22357212 OWN - NLM STAT- MEDLINE DCOM- 20120709 LR - 20211021 IS - 0973-7138 (Electronic) IS - 0250-5991 (Linking) VI - 37 IP - 1 DP - 2012 Mar TI - Molecular cytogenetic identification of a novel dwarf wheat line with introgressed Thinopyrum ponticum chromatin. PG - 149-55 AB - Novel dwarfing germplasms and dwarfing genes are valuable for the wheat breeding. A novel semi-dwarf line, 31505-1, with reduced height compared with its common wheat parent, was derived from a cross between common wheat and Thinopyrum ponticum. Cytological studies demonstrated that 31505-1 contained 42 chromosomes and formed 21 bivalents at meiotic metaphase I. Genomic in situ hybridization (GISH) analysis showed that 31505-1 had no large Th. ponticum chromosome fragments. Fluorescence in situ hybridization (FISH) results revealed the absence of a pAs1 hybridization band on 2DL chromosome of 31505-1. Two SSR markers (Xwmc41 and Xcfd168) and two STS markers (Xmag4059 and Xmag3596), which were located on 2D chromosome, amplified unique bands of Th. Ponticum in 31505-1. These revealed presence of an introgressed Th. ponticum segment in 2DL chromosome of dwarf line 31505-1, although the alien segment could not be detected by GISH. FAU - Chen, Guiling AU - Chen G AD - State Key Laboratory of Crop Biology, Shandong Key Laboratory of Crop Biology, Tai'an Subcenter of National Wheat Improvement Center, Agronomy College, Shandong Agricultural University, Taian 271018, Shandong, China. FAU - Zheng, Qi AU - Zheng Q FAU - Bao, Yinguang AU - Bao Y FAU - Liu, Shubing AU - Liu S FAU - Wang, Honggang AU - Wang H FAU - Li, Xingfeng AU - Li X LA - eng PT - Journal Article PL - India TA - J Biosci JT - Journal of biosciences JID - 8100809 RN - 0 (Chromatin) RN - 0 (Genetic Markers) SB - IM MH - Chromatin/*genetics MH - Crosses, Genetic MH - Cytogenetic Analysis MH - Genetic Markers/genetics MH - Genetics, Population MH - In Situ Hybridization MH - In Situ Hybridization, Fluorescence MH - Poaceae/*genetics MH - Species Specificity MH - Triticum/*genetics/growth & development EDAT- 2012/02/24 06:00 MHDA- 2012/07/10 06:00 CRDT- 2012/02/24 06:00 PHST- 2012/02/24 06:00 [entrez] PHST- 2012/02/24 06:00 [pubmed] PHST- 2012/07/10 06:00 [medline] AID - 10.1007/s12038-011-9175-1 [doi] PST - ppublish SO - J Biosci. 2012 Mar;37(1):149-55. doi: 10.1007/s12038-011-9175-1.