PMID- 22357271
OWN - NLM
STAT- MEDLINE
DCOM- 20120618
LR  - 20211021
IS  - 1098-5514 (Electronic)
IS  - 0022-538X (Print)
IS  - 0022-538X (Linking)
VI  - 86
IP  - 9
DP  - 2012 May
TI  - Human CD4+ T cell response to human herpesvirus 6.
PG  - 4776-92
LID - 10.1128/JVI.06573-11 [doi]
AB  - Following primary infection, human herpesvirus 6 (HHV-6) establishes a persistent 
      infection for life. HHV-6 reactivation has been associated with transplant 
      rejection, delayed engraftment, encephalitis, muscular dystrophy, and 
      drug-induced hypersensitivity syndrome. The poor understanding of the targets and 
      outcome of the cellular immune response to HHV-6 makes it difficult to outline 
      the role of HHV-6 in human disease. To fill in this gap, we characterized CD4 T 
      cell responses to HHV-6 using peripheral blood mononuclear cell (PBMC) and T cell 
      lines generated from healthy donors. CD4(+) T cells responding to HHV-6 in 
      peripheral blood were observed at frequencies below 0.1% of total T cells but 
      could be expanded easily in vitro. Analysis of cytokines in supernatants of PBMC 
      and T cell cultures challenged with HHV-6 preparations indicated that gamma 
      interferon (IFN-gamma) and interleukin-10 (IL-10) were appropriate markers of the 
      HHV-6 cellular response. Eleven CD4(+) T cell epitopes, all but one derived from 
      abundant virion components, were identified. The response was highly 
      cross-reactive between HHV-6A and HHV-6B variants. Seven of the CD4(+) T cell 
      epitopes do not share significant homologies with other known human pathogens, 
      including the closely related human viruses human herpesvirus 7 (HHV-7) and human 
      cytomegalovirus (HCMV). Major histocompatibility complex (MHC) tetramers 
      generated with these epitopes were able to detect HHV-6-specific T cell 
      populations. These findings provide a window into the immune response to HHV-6 
      and provide a basis for tracking HHV-6 cellular immune responses.
FAU - Nastke, Maria-D
AU  - Nastke MD
AD  - Department of Pathology, University of Massachusetts Medical School, Worcester, 
      Massachusetts, USA.
FAU - Becerra, Aniuska
AU  - Becerra A
FAU - Yin, Liusong
AU  - Yin L
FAU - Dominguez-Amorocho, Omar
AU  - Dominguez-Amorocho O
FAU - Gibson, Laura
AU  - Gibson L
FAU - Stern, Lawrence J
AU  - Stern LJ
FAU - Calvo-Calle, J Mauricio
AU  - Calvo-Calle JM
LA  - eng
GR  - P30 DK032520/DK/NIDDK NIH HHS/United States
GR  - DK32520/DK/NIDDK NIH HHS/United States
GR  - AI-U19-57319/AI/NIAID NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
DEP - 20120222
PL  - United States
TA  - J Virol
JT  - Journal of virology
JID - 0113724
RN  - 0 (Antigens, Viral)
RN  - 0 (Cytokines)
RN  - 0 (Epitopes, T-Lymphocyte)
RN  - 0 (HLA-DR1 Antigen)
RN  - 0 (Peptides)
RN  - 82115-62-6 (Interferon-gamma)
SB  - IM
MH  - Antigens, Viral/immunology
MH  - CD4-Positive T-Lymphocytes/*immunology
MH  - Cell Line
MH  - Cross Reactions/immunology
MH  - Cytokines/metabolism
MH  - Epitopes, T-Lymphocyte/immunology
MH  - HLA-DR1 Antigen/chemistry/immunology
MH  - Haplotypes
MH  - Herpesvirus 6, Human/*immunology
MH  - Humans
MH  - Interferon-gamma/metabolism
MH  - Leukocytes, Mononuclear/immunology
MH  - Peptides/immunology
MH  - Protein Multimerization/immunology
MH  - Roseolovirus Infections/immunology/virology
MH  - T-Lymphocytes/immunology
MH  - T-Lymphocytes, Cytotoxic/immunology
MH  - Tissue Donors
MH  - Viral Load
PMC - PMC3347333
EDAT- 2012/02/24 06:00
MHDA- 2012/06/19 06:00
PMCR- 2012/11/01
CRDT- 2012/02/24 06:00
PHST- 2012/02/24 06:00 [entrez]
PHST- 2012/02/24 06:00 [pubmed]
PHST- 2012/06/19 06:00 [medline]
PHST- 2012/11/01 00:00 [pmc-release]
AID - JVI.06573-11 [pii]
AID - 06573-11 [pii]
AID - 10.1128/JVI.06573-11 [doi]
PST - ppublish
SO  - J Virol. 2012 May;86(9):4776-92. doi: 10.1128/JVI.06573-11. Epub 2012 Feb 22.