PMID- 22359062
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20121002
LR  - 20211021
IS  - 0920-9069 (Print)
IS  - 1573-0778 (Electronic)
IS  - 0920-9069 (Linking)
VI  - 29
IP  - 2
DP  - 1999 Mar
TI  - A microcarrier-based cell culture process for the production of a bovine 
      respiratory syncytial virus vaccine.
PG  - 135-49
LID - 10.1023/A:1008022828736 [doi]
AB  - Veterinary viral vaccines generally comprise either attenuated or chemically 
      inactivated viruses which have been propagated on mammalian cell substrates or 
      specific pathogen free (SPF) eggs. New generation vaccines include chemically 
      inactivated virally-infected whole cell vaccines. The NM57 cell line is a bovine 
      nasal turbinate persistently infected (non-lytic infection) with a strain of the 
      respiratory syncytial virus (RSV). The potential of microcarrier technology for 
      the cultivation in bioreactors of this anchorage dependent cell line for RSV 
      vaccine production has been investigated. Both Cytodex 3 and Cultispher S 
      microcarriers proved most suitable from a selection of microcarriers as growth 
      substrates for this NM57 cell line. Maximum cell densities of 4.12x105 cells 
      ml-1and 5.52x105 cells ml-1 respectively were obtained using Cytodex 3 (3 g l-1) 
      and and Cultispher S (1 g l-1) in 5 l bioreactor cultures. The fact that cell 
      growth was less sensitive to agitation rate when cultured on Cultispher S 
      microcarriers, and that cells were efficiently harvested from this microcarrier 
      by an enzymatic method, suggested Cultispher S is suitable for further evaluation 
      at larger bioreactor scales (>5 l) than that described here.
FAU - Moran, E
AU  - Moran E
AD  - MFM Laboratories Ltd., 4 Warner Dr., Springwood Industrial Estate, Braintree, 
      Essex, CM7 2YW, UK.
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Cytotechnology
JT  - Cytotechnology
JID - 8807027
PMC - PMC3449907
EDAT- 1999/03/01 00:00
MHDA- 1999/03/01 00:01
PMCR- 2000/03/01
CRDT- 2012/02/24 06:00
PHST- 2012/02/24 06:00 [entrez]
PHST- 1999/03/01 00:00 [pubmed]
PHST- 1999/03/01 00:01 [medline]
PHST- 2000/03/01 00:00 [pmc-release]
AID - 193329 [pii]
AID - 10.1023/A:1008022828736 [doi]
PST - ppublish
SO  - Cytotechnology. 1999 Mar;29(2):135-49. doi: 10.1023/A:1008022828736.