PMID- 22359676
OWN - NLM
STAT- MEDLINE
DCOM- 20120803
LR  - 20211021
IS  - 1932-6203 (Electronic)
IS  - 1932-6203 (Linking)
VI  - 7
IP  - 2
DP  - 2012
TI  - Antifibrinolytic role of a bee venom serine protease inhibitor that acts as a 
      plasmin inhibitor.
PG  - e32269
LID - 10.1371/journal.pone.0032269 [doi]
LID - e32269
AB  - Bee venom is a rich source of pharmacologically active substances. In this study, 
      we identified a bumblebee (Bombus ignitus) venom Kunitz-type serine protease 
      inhibitor (Bi-KTI) that acts as a plasmin inhibitor. Bi-KTI showed no detectable 
      inhibitory effect on factor Xa, thrombin, or tissue plasminogen activator. In 
      contrast, Bi-KTI strongly inhibited plasmin, indicating that it acts as an 
      antifibrinolytic agent; however, this inhibitory ability was two-fold weaker than 
      that of aprotinin. The fibrin(ogen)olytic activities of B. ignitus venom serine 
      protease (Bi-VSP) and plasmin in the presence of Bi-KTI indicate that Bi-KTI 
      targets plasmin more specifically than Bi-VSP. These findings demonstrate a novel 
      mechanism by which bumblebee venom affects the hemostatic system through the 
      antifibrinolytic activity of Bi-KTI and through Bi-VSP-mediated 
      fibrin(ogen)olytic activities, raising interest in Bi-KTI and Bi-VSP as potential 
      clinical agents.
FAU - Choo, Young Moo
AU  - Choo YM
AD  - College of Natural Resources and Life Science, Dong-A University, Busan, Republic 
      of Korea.
FAU - Lee, Kwang Sik
AU  - Lee KS
FAU - Yoon, Hyung Joo
AU  - Yoon HJ
FAU - Qiu, Yuling
AU  - Qiu Y
FAU - Wan, Hu
AU  - Wan H
FAU - Sohn, Mi Ri
AU  - Sohn MR
FAU - Sohn, Hung Dae
AU  - Sohn HD
FAU - Jin, Byung Rae
AU  - Jin BR
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20120216
PL  - United States
TA  - PLoS One
JT  - PloS one
JID - 101285081
RN  - 0 (Antifibrinolytic Agents)
RN  - 0 (Bee Venoms)
RN  - 0 (Fibrinolytic Agents)
RN  - 0 (Serine Proteinase Inhibitors)
RN  - EC 3.4.21.7 (Fibrinolysin)
SB  - IM
MH  - Animals
MH  - Antifibrinolytic Agents/*isolation & purification
MH  - Bee Venoms/*chemistry
MH  - Bees/*chemistry
MH  - Fibrinolysin/*antagonists & inhibitors
MH  - Fibrinolytic Agents
MH  - Hemostasis
MH  - Humans
MH  - Serine Proteinase Inhibitors/isolation & purification/*pharmacology
PMC - PMC3281132
COIS- Competing Interests: The authors have declared that no competing interests exist.
EDAT- 2012/02/24 06:00
MHDA- 2012/08/04 06:00
PMCR- 2012/02/16
CRDT- 2012/02/24 06:00
PHST- 2011/10/11 00:00 [received]
PHST- 2012/01/24 00:00 [accepted]
PHST- 2012/02/24 06:00 [entrez]
PHST- 2012/02/24 06:00 [pubmed]
PHST- 2012/08/04 06:00 [medline]
PHST- 2012/02/16 00:00 [pmc-release]
AID - PONE-D-11-20017 [pii]
AID - 10.1371/journal.pone.0032269 [doi]
PST - ppublish
SO  - PLoS One. 2012;7(2):e32269. doi: 10.1371/journal.pone.0032269. Epub 2012 Feb 16.