PMID- 22360314
OWN - NLM
STAT- MEDLINE
DCOM- 20130326
LR  - 20220409
IS  - 1582-4934 (Electronic)
IS  - 1582-1838 (Print)
IS  - 1582-1838 (Linking)
VI  - 16
IP  - 10
DP  - 2012 Oct
TI  - Up-regulation of miR-210 by vascular endothelial growth factor in ex vivo 
      expanded CD34+ cells enhances cell-mediated angiogenesis.
PG  - 2413-21
LID - 10.1111/j.1582-4934.2012.01557.x [doi]
AB  - Ex vivo culture has been proposed as a means to augment and repair autologous 
      cells in patients with chronic diseases, but the mechanisms governing improvement 
      in cell function are not well understood. Although microRNAs (miRs) are 
      increasingly appreciated as key regulators of cellular function, a role for these 
      factors in CD34+ cell-mediated angiogenesis has not been elucidated. Vascular 
      endothelial growth factor (VEGF) was previously shown to induce expression of 
      certain miRs associated with angiogenesis in endothelial cells and promote 
      survival and number of vascular colony forming units of haematopoietic stem cells 
      (HSCs). We sought to evaluate the role of VEGF in expansion and angiogenic 
      function of CD34+ cells and to identify specific miRs associated with angiogenic 
      properties of expanded cells. Umbilical cord blood CD34+ cells were effectively 
      expanded (18- to 22-fold) in culture medium containing stem cell factor (SCF), 
      Flt-3 ligand (Flt-3), thrombopoietin (TPO) and interleukin-6 (IL-6) with 
      (postEX/+VEGF) and without VEGF (postEX/noVEGF). Tube formation in matrigel assay 
      and tissue perfusion/capillary density in mice ischaemic hindlimb were 
      significantly improved by postEX/+VEGF cells compared with fresh CD34+ and 
      postEX/noVEGF cells. MiR-210 expression was significantly up-regulated in 
      postEX/+VEGF cells. MiR-210 inhibitor abrogated and 210 mimic recapitulated the 
      pro-angiogenic effects by treatment of postEX/+VEGF and postEX/noVEGF cells 
      respectively. Collectively, these observations highlight a critical role for VEGF 
      in enhancing the angiogenic property of expanded cells, and identify miR-210 as a 
      potential therapeutic target to enhance CD34+ stem cell function for the 
      treatment of ischaemic vascular disease.
CI  - (c) 2012 The Authors Journal of Cellular and Molecular Medicine (c) 2012 Foundation 
      for Cellular and Molecular Medicine/Blackwell Publishing Ltd.
FAU - Alaiti, Mohamad Amer
AU  - Alaiti MA
AD  - Department of Medicine, Case Cardiovascular Research Institute, University 
      Hospital Case Medical Center, Case Western Reserve University School of Medicine, 
      Cleveland, OH 44106-5038, USA.
FAU - Ishikawa, Masakazu
AU  - Ishikawa M
FAU - Masuda, Haruchika
AU  - Masuda H
FAU - Simon, Daniel I
AU  - Simon DI
FAU - Jain, Mukesh K
AU  - Jain MK
FAU - Asahara, Takayuki
AU  - Asahara T
FAU - Costa, Marco A
AU  - Costa MA
LA  - eng
GR  - P30 CA043703/CA/NCI NIH HHS/United States
GR  - R37 HL057506/HL/NHLBI NIH HHS/United States
GR  - P30 CA43703/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - J Cell Mol Med
JT  - Journal of cellular and molecular medicine
JID - 101083777
RN  - 0 (Angiogenesis Inducing Agents)
RN  - 0 (Antigens, CD34)
RN  - 0 (Interleukin-6)
RN  - 0 (MIRN210 microRNA, human)
RN  - 0 (Membrane Proteins)
RN  - 0 (MicroRNAs)
RN  - 0 (Stem Cell Factor)
RN  - 0 (VEGFA protein, human)
RN  - 0 (Vascular Endothelial Growth Factor A)
RN  - 0 (flt3 ligand protein)
RN  - 9014-42-0 (Thrombopoietin)
SB  - IM
MH  - Angiogenesis Inducing Agents/*metabolism
MH  - Animals
MH  - Antigens, CD34/metabolism
MH  - Fetal Blood/cytology
MH  - Gene Expression Regulation
MH  - Gene Silencing
MH  - Human Umbilical Vein Endothelial Cells
MH  - Humans
MH  - Interleukin-6/genetics/metabolism
MH  - Male
MH  - Membrane Proteins/genetics/metabolism
MH  - Mice
MH  - Mice, Inbred NOD
MH  - Mice, SCID
MH  - MicroRNAs/genetics/*metabolism
MH  - *Neovascularization, Physiologic
MH  - Stem Cell Factor
MH  - Thrombopoietin/genetics/metabolism
MH  - Up-Regulation
MH  - Vascular Endothelial Growth Factor A/genetics/*metabolism
PMC - PMC3823435
MID - NIHMS596569
EDAT- 2012/03/01 06:00
MHDA- 2013/03/27 06:00
PMCR- 2012/10/01
CRDT- 2012/02/25 06:00
PHST- 2012/02/25 06:00 [entrez]
PHST- 2012/03/01 06:00 [pubmed]
PHST- 2013/03/27 06:00 [medline]
PHST- 2012/10/01 00:00 [pmc-release]
AID - 10.1111/j.1582-4934.2012.01557.x [doi]
PST - ppublish
SO  - J Cell Mol Med. 2012 Oct;16(10):2413-21. doi: 10.1111/j.1582-4934.2012.01557.x.