PMID- 22361885
OWN - NLM
STAT- MEDLINE
DCOM- 20120618
LR  - 20191112
IS  - 1880-313X (Electronic)
IS  - 0388-6107 (Linking)
VI  - 33
IP  - 1
DP  - 2012 Feb
TI  - Toll like receptor 5 ligand induces monocyte chemoattractant protein-1 in mouse 
      osteoblastic cells.
PG  - 39-44
AB  - Flagellin, the ligand of Toll like receptor 5, is the major subunit of bacterial 
      flagella. Flagellin stimulates various cells to release chemokines. Monocyte 
      chemoattractant protein-1 (MCP-1) is a member of the CC chemokine family that is 
      involved in monocyte infiltration in inflammatory diseases. It has been reported 
      that serum MCP-1 levels increase proportionally with the severity of periodontal 
      disease. Inflammatory mediators induce MCP-1 production in various cells, 
      including osteoblasts. However, it remains unclear whether MCP-1 is released from 
      osteoblasts in response to flagellin. In the present study, we investigated the 
      effects of flagellin on the expression of MCP-1 in the mouse osteoblastic cell 
      line, MC3T3-E1 (E1) cells. Flagellin markedly increased MCP-1 mRNA level in a 
      dose-dependent manner. The effect of flagellin on MCP-1 mRNA expression in E1 
      cells was transient, with a peak at 1 h. Concomitant with MCP-1 mRNA expression, 
      MCP-1 protein levels were clearly elevated at 3 h after flagellin exposure. In 
      addition, we revealed that JNK and MEK-ERK1/2 are involved in flagellin-induced 
      MCP-1 expression in E1 cells. These results indicated that bacterial flagellin 
      may play an important role in the progression of periodontitis. Results of 
      further studies will provide more clues to the prevention of periodontal 
      diseases.
FAU - Nakamura, Kimiya
AU  - Nakamura K
AD  - Comprehensive Conservative Dentistry, Hokkaido University Hospital, Kita 14 Nishi 
      5, Kita-ku, Sapporo 060-8648, Japan.
FAU - Deyama, Yoshiaki
AU  - Deyama Y
FAU - Yoshimura, Yoshitaka
AU  - Yoshimura Y
FAU - Suzuki, Kuniaki
AU  - Suzuki K
FAU - Morita, Manabu
AU  - Morita M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Japan
TA  - Biomed Res
JT  - Biomedical research (Tokyo, Japan)
JID - 8100317
RN  - 0 (Ccl2 protein, mouse)
RN  - 0 (Chemokine CCL2)
RN  - 0 (RNA, Messenger)
RN  - 0 (Toll-Like Receptor 5)
RN  - 12777-81-0 (Flagellin)
SB  - IM
MH  - Animals
MH  - Cell Line
MH  - Chemokine CCL2/genetics/*metabolism
MH  - Flagellin/*pharmacology
MH  - Mice
MH  - Osteoblasts/*cytology/*metabolism
MH  - Periodontal Diseases/drug therapy
MH  - RNA, Messenger/genetics
MH  - Signal Transduction
MH  - Toll-Like Receptor 5/metabolism
EDAT- 2012/03/01 06:00
MHDA- 2012/06/19 06:00
CRDT- 2012/02/25 06:00
PHST- 2012/02/25 06:00 [entrez]
PHST- 2012/03/01 06:00 [pubmed]
PHST- 2012/06/19 06:00 [medline]
AID - JST.JSTAGE/biomedres/33.39 [pii]
AID - 10.2220/biomedres.33.39 [doi]
PST - ppublish
SO  - Biomed Res. 2012 Feb;33(1):39-44. doi: 10.2220/biomedres.33.39.