PMID- 22363931
OWN - NLM
STAT- MEDLINE
DCOM- 20120726
LR  - 20120305
IS  - 1364-5528 (Electronic)
IS  - 0003-2654 (Linking)
VI  - 137
IP  - 7
DP  - 2012 Apr 7
TI  - Analysis of plant hormones by microemulsion electrokinetic capillary 
      chromatography coupled with on-line large volume sample stacking.
PG  - 1723-9
LID - 10.1039/c2an16313f [doi]
AB  - A novel method of microemulsion electrokinetic capillary chromatography (MEEKC) 
      coupled with on-line large volume sample stacking was developed for the analysis 
      of six plant hormones including indole-3-acetic acid, indole-3-butyric acid, 
      indole-3-propionic acid, 1-naphthaleneacetic acid, abscisic acid and salicylic 
      acid. Baseline separation of six plant hormones was achieved within 10 min by 
      using the microemulsion background electrolyte containing a 97.2% (w/w) 10 mM 
      borate buffer at pH 9.2, 1.0% (w/w) ethyl acetate as oil droplets, 0.6% (w/w) 
      sodium dodecyl sulphate as surfactant and 1.2% (w/w) 1-butanol as cosurfactant. 
      In addition, an on-line concentration method based on a large volume sample 
      stacking technique and multiple wavelength detection was adopted for improving 
      the detection sensitivity in order to determine trace level hormones in a real 
      sample. The optimal method provided about 50-100 fold increase in detection 
      sensitivity compared with a single MEEKC method, and the detection limits (S/N = 
      3) were between 0.005 and 0.02 mug mL(-1). The proposed method was simple, rapid 
      and sensitive and could be applied to the determination of six plant hormones in 
      spiked water samples, tobacco leaves and 1-naphthylacetic acid in leaf 
      fertilizer. The recoveries ranged from 76.0% to 119.1%, and good 
      reproducibilities were obtained with relative standard deviations (RSDs) less 
      than 6.6%.
FAU - Chen, Zongbao
AU  - Chen Z
AD  - Ministry of Education Key Laboratory of Analysis and Detection for Food Safety, 
      Department of Chemistry, Fuzhou University, Fuzhou 350002, Fujian, China.
FAU - Lin, Zian
AU  - Lin Z
FAU - Zhang, Lin
AU  - Zhang L
FAU - Cai, Yan
AU  - Cai Y
FAU - Zhang, Lan
AU  - Zhang L
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20120223
PL  - England
TA  - Analyst
JT  - The Analyst
JID - 0372652
RN  - 0 (Plant Growth Regulators)
SB  - IM
MH  - Chemistry Techniques, Analytical/*methods
MH  - *Chromatography, Micellar Electrokinetic Capillary
MH  - Molecular Structure
MH  - Plant Growth Regulators/*analysis/chemistry
EDAT- 2012/03/01 06:00
MHDA- 2012/07/27 06:00
CRDT- 2012/02/25 06:00
PHST- 2012/02/25 06:00 [entrez]
PHST- 2012/03/01 06:00 [pubmed]
PHST- 2012/07/27 06:00 [medline]
AID - 10.1039/c2an16313f [doi]
PST - ppublish
SO  - Analyst. 2012 Apr 7;137(7):1723-9. doi: 10.1039/c2an16313f. Epub 2012 Feb 23.