PMID- 22364688 OWN - NLM STAT- MEDLINE DCOM- 20120730 LR - 20211021 IS - 1873-6823 (Electronic) IS - 0741-8329 (Print) IS - 0741-8329 (Linking) VI - 46 IP - 3 DP - 2012 May TI - Serum and plasma brain-derived neurotrophic factor (BDNF) in abstinent alcoholics and social drinkers. PG - 253-9 LID - 10.1016/j.alcohol.2011.12.001 [doi] AB - Although the effects of alcohol on brain-derived neurotrophic factor (BDNF) have been extensively studied in rodents, BDNF levels have rarely been measured in abstinent, alcohol-dependent (AD) individuals. Interpretation of reported group comparisons of serum BDNF levels is difficult due to limited information regarding analytical variance, biological variability, and the relative contribution of platelet and plasma pools to serum BDNF. Analytical variance (intra- and inter-assay coefficients of variation) of the enzyme-linked immunosorbent assay (ELISA) was characterized. Within- and between-subject variability, and group differences in serum and plasma BDNF, was assessed on three separate days in 16, 4-week abstinent AD individuals (7M/9F) and 16 social drinkers (SDs; 8M/8F). Significantly higher mean (+/-sd) serum BDNF levels were observed for the AD group compared to the SD (p = 0.003). No significant difference in mean baseline plasma BDNF levels was observed between AD and SD groups. The low analytical variance, high day-to-day within-individual stability and the high degree of individuality demonstrates the potential clinical utility of measuring serum BDNF levels. The low correlations that we observed between plasma and serum levels are congruent with their representing separate pools of BDNF. The observation of higher basal serum BDNF in the AD group without a concomitant elevation in plasma BDNF levels indicates that the elevated serum BDNF in AD patients is not due to greater BDNF exposure. Further research is warranted to fully elucidate mechanisms underlying this alteration and determine the utility of serum BDNF as a predictor or surrogate marker of chronic alcohol abuse. CI - Copyright (c) 2012 Elsevier Inc. All rights reserved. FAU - D'Sa, Carrol AU - D'Sa C AD - Yale Stress Center, Department of Psychiatry, Yale University School of Medicine, New Haven, CT 06519, USA. carrol.dsa@yale.edu FAU - Dileone, Ralph J AU - Dileone RJ FAU - Anderson, George M AU - Anderson GM FAU - Sinha, Rajita AU - Sinha R LA - eng GR - R01 AA013892/AA/NIAAA NIH HHS/United States GR - UL1 DE019586/DE/NIDCR NIH HHS/United States GR - R01-AA013892/AA/NIAAA NIH HHS/United States GR - PL1-DA024859/DA/NIDA NIH HHS/United States GR - PL1 DA024860/DA/NIDA NIH HHS/United States GR - PL1 DA024859/DA/NIDA NIH HHS/United States PT - Journal Article PT - Research Support, N.I.H., Extramural DEP - 20120222 PL - United States TA - Alcohol JT - Alcohol (Fayetteville, N.Y.) JID - 8502311 RN - 0 (Brain-Derived Neurotrophic Factor) SB - IM MH - Adult MH - Alcohol Drinking/*blood MH - Alcoholics MH - Alcoholism/*blood MH - Brain-Derived Neurotrophic Factor/*blood MH - Female MH - Humans MH - Male MH - Middle Aged MH - Plasma/chemistry MH - Serum/chemistry MH - *Temperance PMC - PMC3674784 MID - NIHMS471597 EDAT- 2012/03/01 06:00 MHDA- 2012/07/31 06:00 PMCR- 2013/06/06 CRDT- 2012/02/28 06:00 PHST- 2010/10/21 00:00 [received] PHST- 2011/09/19 00:00 [revised] PHST- 2011/12/14 00:00 [accepted] PHST- 2012/02/28 06:00 [entrez] PHST- 2012/03/01 06:00 [pubmed] PHST- 2012/07/31 06:00 [medline] PHST- 2013/06/06 00:00 [pmc-release] AID - S0741-8329(11)00522-2 [pii] AID - 10.1016/j.alcohol.2011.12.001 [doi] PST - ppublish SO - Alcohol. 2012 May;46(3):253-9. doi: 10.1016/j.alcohol.2011.12.001. Epub 2012 Feb 22.