PMID- 22364913
OWN - NLM
STAT- MEDLINE
DCOM- 20120830
LR  - 20211021
IS  - 1096-0023 (Electronic)
IS  - 1043-4666 (Linking)
VI  - 58
IP  - 2
DP  - 2012 May
TI  - Reconstituting National Institute for Biological Standards and Control (NIBSC) 
      chemokines.
PG  - 162-4
LID - 10.1016/j.cyto.2012.01.016 [doi]
AB  - We observed significant discrepancies between immunoassay results when using 
      different internally prepared reference preparations for interleukin-8 (IL-8) and 
      monocyte chemoattractant protein-1 (MCP-1) from the National Institute for 
      Biological Standards and Control (NIBSC). To evaluate the reasons for this we 
      prepared the chemokines using diluents that incorporated protein at different 
      steps. This showed that even brief addition of water to these preparations, in 
      the absence of additional protein, resulted in loss of immunoreactivity in 
      assays. The data obtained emphasise the importance of adding protein at an early 
      stage of preparation to avoid loss of material and potential loss of activity.
CI  - Copyright (c) 2012 Elsevier Ltd. All rights reserved.
FAU - Hoadley, Margaret E
AU  - Hoadley ME
AD  - Brain Injury Research Group, Clinical Sciences Building, Salford Royal Foundation 
      Trust, Salford M6 8HD, UK. margaret.hoadley@manchester.ac.uk
FAU - Scarth, Sylvia
AU  - Scarth S
FAU - Hopkins, Stephen J
AU  - Hopkins SJ
LA  - eng
GR  - G0502030/MRC_/Medical Research Council/United Kingdom
GR  - G0801040/MRC_/Medical Research Council/United Kingdom
PT  - Journal Article
DEP - 20120224
PL  - England
TA  - Cytokine
JT  - Cytokine
JID - 9005353
RN  - 0 (Chemokine CCL2)
RN  - 0 (Chemokines)
RN  - 0 (Interleukin-8)
SB  - IM
MH  - Chemokine CCL2/standards
MH  - Chemokines/*standards
MH  - Government Agencies
MH  - Humans
MH  - Interleukin-8/standards
MH  - United States
EDAT- 2012/03/01 06:00
MHDA- 2012/08/31 06:00
CRDT- 2012/02/28 06:00
PHST- 2011/12/20 00:00 [received]
PHST- 2012/01/26 00:00 [revised]
PHST- 2012/01/27 00:00 [accepted]
PHST- 2012/02/28 06:00 [entrez]
PHST- 2012/03/01 06:00 [pubmed]
PHST- 2012/08/31 06:00 [medline]
AID - S1043-4666(12)00031-2 [pii]
AID - 10.1016/j.cyto.2012.01.016 [doi]
PST - ppublish
SO  - Cytokine. 2012 May;58(2):162-4. doi: 10.1016/j.cyto.2012.01.016. Epub 2012 Feb 
      24.