PMID- 22366140
OWN - NLM
STAT- MEDLINE
DCOM- 20120802
LR  - 20131121
IS  - 0236-6290 (Print)
IS  - 0236-6290 (Linking)
VI  - 60
IP  - 1
DP  - 2012 Mar
TI  - Genetic diversity analysis of the ORF5 gene in porcine reproductive and 
      respiratory syndrome virus samples from South China.
PG  - 157-64
LID - 10.1556/AVet.2012.013 [doi]
AB  - To understand the genetic diversity of porcine reproductive and respiratory 
      syndrome virus (PRRSV) in South China, we collected 231 clinical samples from 
      pigs with suspected PRRSV infection in Guangdong between 2007 and 2009. We found 
      that 74 of 231 samples were positive by RT-PCR. The PCR products of the ORF5 gene 
      of 35 isolates from different farms were sequenced and their DNA sequences were 
      compared to 23 other PRRSV isolates in the GenBank. We found that the nucleotide 
      similarity among all South China isolates ranged from 87.6% to 100%, and all 
      belonged to the North American genotype. Most of them were classified into 
      subgenotype I, but the rest mapped to subgenotypes III, V or VI. Those in 
      subgenotypes I and III were found to be highly variable in the primary 
      neutralising epitope (PNE) with a specific amino acid mutation (F39/L39-->I39), and 
      a few isolates in subgenotypes I and III isolates also had a mutation at L41 
      (L41-->S41). PRRSV isolates in subgenotypes III, V and VI had less potential 
      glycosylation sites than those in subgenotype I. Our data contribute to the 
      understanding of molecular variation of PRRSV in South China.
FAU - Cao, Zong-Xi
AU  - Cao ZX
AD  - South China Agricultural University College of Veterinary Medicine, Guangzhou, 
      China.
FAU - Jiao, Pei-Rong
AU  - Jiao PR
FAU - Huang, Yu-Mao
AU  - Huang YM
FAU - Qin, Hong-Yang
AU  - Qin HY
FAU - Kong, Liu-Wu
AU  - Kong LW
FAU - Pan, Quan-Hui
AU  - Pan QH
FAU - He, Yi-Min
AU  - He YM
FAU - Zhang, Gui-Hong
AU  - Zhang GH
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Hungary
TA  - Acta Vet Hung
JT  - Acta veterinaria Hungarica
JID - 8406376
RN  - 0 (Viral Envelope Proteins)
RN  - 0 (Viral Proteins)
RN  - 0 (glycoprotein 5, PRRSV)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - China/epidemiology
MH  - Gene Expression Regulation, Viral
MH  - *Genetic Variation
MH  - Molecular Sequence Data
MH  - Phylogeny
MH  - Porcine Reproductive and Respiratory Syndrome/epidemiology/*virology
MH  - Porcine respiratory and reproductive syndrome virus/*genetics
MH  - Swine
MH  - Viral Envelope Proteins
MH  - Viral Proteins/*genetics
EDAT- 2012/03/01 06:00
MHDA- 2012/08/03 06:00
CRDT- 2012/02/28 06:00
PHST- 2012/02/28 06:00 [entrez]
PHST- 2012/03/01 06:00 [pubmed]
PHST- 2012/08/03 06:00 [medline]
AID - B591315442646X52 [pii]
AID - 10.1556/AVet.2012.013 [doi]
PST - ppublish
SO  - Acta Vet Hung. 2012 Mar;60(1):157-64. doi: 10.1556/AVet.2012.013.