PMID- 22369868
OWN - NLM
STAT- MEDLINE
DCOM- 20120808
LR  - 20161125
IS  - 1878-5867 (Electronic)
IS  - 0039-128X (Linking)
VI  - 77
IP  - 6
DP  - 2012 May
TI  - Influences of beta-HCG administration on carbon isotope ratios of endogenous urinary 
      steroids.
PG  - 644-54
LID - 10.1016/j.steroids.2012.02.009 [doi]
AB  - Several factors influencing the carbon isotope ratios (CIR) of endogenous urinary 
      steroids have been identified in recent years. One of these should be the 
      metabolism of steroids inside the body involving numerous different enzymes. A 
      detailed look at this metabolism taking into account differences found between 
      steroids excreted as glucuronides or as sulphates and hydrogen isotope ratios of 
      different steroids pointed out possibility of unequal CIR at the main production 
      sites inside the male body - the testes and the adrenal glands. By administration 
      of beta-HCG it is possible to strongly stimulate the steroid production within the 
      testes without influencing the production at the adrenal glands. Therefore, this 
      treatment should result in changed CIR of urinary androgens in contrast to the 
      undisturbed pre-treatment values. Four male volunteers received three injections 
      of beta-HCG over a time course of 5 days and collected their urine samples at 
      defined intervals after the last administration. Those samples showing the 
      largest response in contrast to the pre-administration urines were identified by 
      steroid profile measurements and subsequent analysed by GC/C/IRMS. CIR of 
      androsterone, etiocholanolone, testosterone, 5alpha- and 5beta-androstanediol and 
      pregnanediol were compared. While pregnanediol was not influenced, most of the 
      investigated androgens showed depleted values after treatment. The majority of 
      differences were found to be statistically significant and nearly all showed the 
      expected trend towards more depleted delta(13)C-values. These results support the 
      hypothesis of different CIR at different production sites inside the human body. 
      The impact of these findings on doping control analysis will be discussed.
CI  - Copyright (c) 2012 Elsevier Inc. All rights reserved.
FAU - Piper, Thomas
AU  - Piper T
AD  - Swiss Laboratory for Doping Analysis, University Center of Legal Medicine, Geneva 
      and Lausanne, Centre Hospitalier Universitaire Vaudois and University Lausanne, 
      Ch. des Croisettes 22, CH-1066 Epalinges, Switzerland. thomas.piper@chuv.ch
FAU - Baume, Norbert
AU  - Baume N
FAU - Strahm, Emanuel
AU  - Strahm E
FAU - Emery, Caroline
AU  - Emery C
FAU - Saugy, Martial
AU  - Saugy M
LA  - eng
PT  - Clinical Trial
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20120217
PL  - United States
TA  - Steroids
JT  - Steroids
JID - 0404536
RN  - 0 (Carbon Isotopes)
RN  - 0 (Chorionic Gonadotropin, beta Subunit, Human)
RN  - 0 (Steroids)
RN  - 0 (Sulfates)
RN  - 97C5T2UQ7J (Cholesterol)
RN  - EC 1.14.14.19 (Steroid 17-alpha-Hydroxylase)
SB  - IM
MH  - Adult
MH  - Carbon Isotopes/analysis
MH  - Cholesterol/urine
MH  - Chorionic Gonadotropin, beta Subunit, Human/*administration & 
      dosage/*pharmacology
MH  - Doping in Sports/prevention & control
MH  - Humans
MH  - Male
MH  - Steroid 17-alpha-Hydroxylase/urine
MH  - Steroids/*chemistry/*urine
MH  - Sulfates/chemistry
MH  - Urinalysis
MH  - Young Adult
EDAT- 2012/03/01 06:00
MHDA- 2012/08/09 06:00
CRDT- 2012/02/29 06:00
PHST- 2012/01/09 00:00 [received]
PHST- 2012/02/08 00:00 [revised]
PHST- 2012/02/09 00:00 [accepted]
PHST- 2012/02/29 06:00 [entrez]
PHST- 2012/03/01 06:00 [pubmed]
PHST- 2012/08/09 06:00 [medline]
AID - S0039-128X(12)00067-0 [pii]
AID - 10.1016/j.steroids.2012.02.009 [doi]
PST - ppublish
SO  - Steroids. 2012 May;77(6):644-54. doi: 10.1016/j.steroids.2012.02.009. Epub 2012 
      Feb 17.