PMID- 22370705
OWN - NLM
STAT- MEDLINE
DCOM- 20130411
LR  - 20211021
IS  - 1414-431X (Electronic)
IS  - 0100-879X (Print)
IS  - 0100-879X (Linking)
VI  - 45
IP  - 3
DP  - 2012 Mar
TI  - Cardiovascular effects of the intracerebroventricular injection of 
      adrenomedullin: roles of the peripheral vasopressin and central cholinergic 
      systems.
PG  - 250-5
LID - S0100-879X2012007500027 [pii]
AB  - Our objective was to investigate in conscious Sprague-Dawley (6-8 weeks, 250-300 
      g) female rats (N = 7 in each group) the effects of intracerebroventricularly 
      (icv) injected adrenomedullin (ADM) on blood pressure and heart rate (HR), and to 
      determine if ADM and calcitonin gene-related peptide (CGRP) receptors, peripheral 
      V1 receptors or the central cholinergic system play roles in these cardiovascular 
      effects. Blood pressure and HR were observed before and for 30 min following drug 
      injections. The following results were obtained: 1) icv ADM (750 ng/10 microL) caused 
      an increase in both blood pressure and HR (DMAP = 11.8 +/- 2.3 mmHg and DeltaHR = 39.7 
      +/- 4.8 bpm). 2) Pretreatment with a CGRP receptor antagonist (CGRP8-37) and ADM 
      receptor antagonist (ADM22-52) blocked the effect of central ADM on blood 
      pressure and HR. 3) The nicotinic receptor antagonist mecamylamine (25 microg/10 microL, 
      icv) and the muscarinic receptor antagonist atropine (5 microg/10 microL, icv) prevented 
      the stimulating effect of ADM on blood pressure. The effect of ADM on HR was 
      blocked only by atropine (5 microg/10 microL, icv). 4) The V1 receptor antagonist 
      [beta-mercapto-beta-beta-cyclopentamethylenepropionyl(1), O-me-Tyr(2),Arg8]-vasopressin 
      (V2255; 10 microg/kg), that was applied intravenously, prevented the effect of ADM on 
      blood pressure and HR. This is the first study reporting the role of specific ADM 
      and CGRP receptors, especially the role of nicotinic and muscarinic central 
      cholinergic receptors and the role of peripheral V1 receptors in the increasing 
      effects of icv ADM on blood pressure and HR.
FAU - Cam-Etoz, B
AU  - Cam-Etoz B
AD  - Department of Physiology, Uludag University Medical Faculty, Gorukle/Bursa, 
      Turkey. mbetul@uludag.edu.tr
FAU - Isbil-Buyukcoskun, N
AU  - Isbil-Buyukcoskun N
FAU - Ozluk, K
AU  - Ozluk K
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20120301
PL  - Brazil
TA  - Braz J Med Biol Res
JT  - Brazilian journal of medical and biological research = Revista brasileira de 
      pesquisas medicas e biologicas
JID - 8112917
RN  - 0 (Receptors, Calcitonin Gene-Related Peptide)
RN  - 0 (Vasodilator Agents)
RN  - 11000-17-2 (Vasopressins)
RN  - 148498-78-6 (Adrenomedullin)
SB  - IM
MH  - Adrenomedullin/administration & dosage/*pharmacology
MH  - Animals
MH  - Blood Pressure/*drug effects
MH  - Central Nervous System/drug effects/physiology
MH  - Cholinergic Neurons/drug effects/*physiology
MH  - Consciousness/drug effects/physiology
MH  - Female
MH  - Heart Rate/*drug effects
MH  - Injections, Intraventricular
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Receptors, Calcitonin Gene-Related Peptide/drug effects/physiology
MH  - Vasodilator Agents/administration & dosage/*pharmacology
MH  - Vasopressins/*drug effects/physiology
PMC - PMC3854196
EDAT- 2012/03/01 06:00
MHDA- 2013/04/12 06:00
PMCR- 2012/03/02
CRDT- 2012/02/29 06:00
PHST- 2011/03/21 00:00 [received]
PHST- 2012/02/13 00:00 [accepted]
PHST- 2012/02/29 06:00 [entrez]
PHST- 2012/03/01 06:00 [pubmed]
PHST- 2013/04/12 06:00 [medline]
PHST- 2012/03/02 00:00 [pmc-release]
AID - S0100-879X2012007500027 [pii]
AID - 10.1590/s0100-879x2012007500027 [doi]
PST - ppublish
SO  - Braz J Med Biol Res. 2012 Mar;45(3):250-5. doi: 10.1590/s0100-879x2012007500027. 
      Epub 2012 Mar 1.