PMID- 22371907
OWN - NLM
STAT- MEDLINE
DCOM- 20120614
LR  - 20120228
IS  - 1550-8080 (Electronic)
IS  - 0091-7370 (Linking)
VI  - 42
IP  - 1
DP  - 2012 Winter
TI  - Genetic ablation of toll-like receptor 2 reduces secondary brain injury caused by 
      cortical contusion in mice.
PG  - 26-33
AB  - Previous studies have shown that Toll-like receptor 2 (TLR2) was up-regulated 
      after traumatic brain injury (TBI), but the potential contribution of TLR2 to TBI 
      still remains unclear. The present study investigated the role of TLR2 in 
      modulating TBI-induced secondary brain injury in mice. Wild-type TLR2(+/+) and 
      TLR2(-/-)-deficient mice were subjected to a moderately severe weight-drop impact 
      head injury. Brain samples were extracted at 24 hours after trauma. We measured 
      TLR2 by western blot; motor function by Grip test; brain edema by wet/dry method; 
      cortical apoptosis by terminal deoxynucleotidyl transferase-mediated dUTP nick 
      end labeling (TUNEL) method; and IL-1beta, TNF-alpha and IL-6 by enzyme-linked 
      immunosorbent assay (ELISA). We found the absence of TLR2 function in mice 
      resulted in amelio-rating brain injury as shown by the reduced severity of 
      neurological deficit, apoptosis, and brain edema at 24 hours after TBI, which was 
      associated with the decreased expression of inflammatory cytokines such as tumor 
      necrosis factor-alpha (TNF-alpha), interleukin-1beta (IL-1beta), and interleukin-6 (IL-6), 
      compared with their wild-type counterparts after TBI. In combination, these 
      results suggest that TLR2 might play an important aggravating role in the 
      pathogenesis of TBI-induced secondary brain injury, possibly by regulating 
      inflammatory cytokines in the cortex.
FAU - Yu, Zheng-Quan
AU  - Yu ZQ
AD  - Jiangsu Province, China. yuzhengquan1964@gmail.com
FAU - Zha, Ji-Hui
AU  - Zha JH
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Ann Clin Lab Sci
JT  - Annals of clinical and laboratory science
JID - 0410247
RN  - 0 (Cytokines)
RN  - 0 (Inflammation Mediators)
RN  - 0 (Tlr2 protein, mouse)
RN  - 0 (Toll-Like Receptor 2)
SB  - IM
MH  - Animals
MH  - Apoptosis
MH  - Blotting, Western
MH  - Brain Edema/complications/metabolism/pathology/physiopathology
MH  - Brain Injuries/complications/*pathology/physiopathology/*prevention & control
MH  - Cerebral Cortex/*injuries/pathology/physiopathology
MH  - Cytokines/metabolism
MH  - *Gene Deletion
MH  - Hand Strength
MH  - Immunohistochemistry
MH  - In Situ Nick-End Labeling
MH  - Inflammation Mediators/metabolism
MH  - Mice
MH  - Mice, Inbred ICR
MH  - Toll-Like Receptor 2/*deficiency/genetics
EDAT- 2012/03/01 06:00
MHDA- 2012/06/15 06:00
CRDT- 2012/02/29 06:00
PHST- 2012/02/29 06:00 [entrez]
PHST- 2012/03/01 06:00 [pubmed]
PHST- 2012/06/15 06:00 [medline]
AID - 42/1/26 [pii]
PST - ppublish
SO  - Ann Clin Lab Sci. 2012 Winter;42(1):26-33.