PMID- 2237408
OWN - NLM
STAT- MEDLINE
DCOM- 19901206
LR  - 20190618
IS  - 0036-8075 (Print)
IS  - 0036-8075 (Linking)
VI  - 250
IP  - 4980
DP  - 1990 Oct 26
TI  - Detection of bcr-abl fusion in chronic myelogeneous leukemia by in situ 
      hybridization.
PG  - 559-62
AB  - Chronic myelogeneous leukemia (CML) is genetically characterized by fusion of the 
      bcr and abl genes on chromosomes 22 and 9, respectively. In most cases, the 
      fusion involves a reciprocal translocation t(9;22)(q34;q11), which produces the 
      cytogenetically distinctive Philadelphia chromosome (Ph1). Fusion can be detected 
      by Southern (DNA) analysis or by in vitro amplification of the messenger RNA from 
      the fusion gene with polymerase chain reaction (PCR). These techniques are 
      sensitive but cannot be applied to single cells. Two-color fluorescence in situ 
      hybridization (FISH) was used with probes from portions of the bcr and abl genes 
      to detect the bcr-abl fusion in individual blood and bone marrow cells from six 
      patients. The fusion event was detected in all samples analyzed, of which three 
      were cytogenetically Ph1-negative. One of the Ph1-negative samples was also 
      PCR-negative. This approach is fast and sensitive, and provides potential for 
      determining the frequency of the abnormality in different cell lineages.
FAU - Tkachuk, D C
AU  - Tkachuk DC
AD  - Lawrence-Livermore National Laboratory, Biomedical and Environmental Sciences 
      Division, CA 94550.
FAU - Westbrook, C A
AU  - Westbrook CA
FAU - Andreeff, M
AU  - Andreeff M
FAU - Donlon, T A
AU  - Donlon TA
FAU - Cleary, M L
AU  - Cleary ML
FAU - Suryanarayan, K
AU  - Suryanarayan K
FAU - Homge, M
AU  - Homge M
FAU - Redner, A
AU  - Redner A
FAU - Gray, J
AU  - Gray J
FAU - Pinkel, D
AU  - Pinkel D
LA  - eng
GR  - CA 45919/CA/NCI NIH HHS/United States
GR  - CA44700/CA/NCI NIH HHS/United States
GR  - CA49605/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Science
JT  - Science (New York, N.Y.)
JID - 0404511
RN  - 0 (Proto-Oncogene Proteins)
RN  - EC 2.7.10.1 (Protein-Tyrosine Kinases)
RN  - EC 2.7.10.2 (Fusion Proteins, bcr-abl)
RN  - EC 2.7.11.1 (BCR protein, human)
RN  - EC 2.7.11.1 (Proto-Oncogene Proteins c-bcr)
SB  - IM
GS  - abl
GS  - bcr
MH  - Chromosomes, Human, Pair 22
MH  - Chromosomes, Human, Pair 9
MH  - Fusion Proteins, bcr-abl/*genetics
MH  - Genes, abl
MH  - Humans
MH  - Interphase
MH  - Leukemia, Myelogenous, Chronic, BCR-ABL Positive/*genetics
MH  - Metaphase
MH  - Nucleic Acid Hybridization
MH  - Philadelphia Chromosome
MH  - *Protein-Tyrosine Kinases
MH  - Proto-Oncogene Proteins/genetics
MH  - Proto-Oncogene Proteins c-bcr
MH  - Translocation, Genetic
EDAT- 1990/10/26 00:00
MHDA- 1990/10/26 00:01
CRDT- 1990/10/26 00:00
PHST- 1990/10/26 00:00 [pubmed]
PHST- 1990/10/26 00:01 [medline]
PHST- 1990/10/26 00:00 [entrez]
AID - 10.1126/science.2237408 [doi]
PST - ppublish
SO  - Science. 1990 Oct 26;250(4980):559-62. doi: 10.1126/science.2237408.