PMID- 22376040
OWN - NLM
STAT- MEDLINE
DCOM- 20120504
LR  - 20221207
IS  - 1532-4303 (Electronic)
IS  - 0277-0903 (Linking)
VI  - 49
IP  - 3
DP  - 2012 Apr
TI  - Gene-gene interactions between candidate gene polymorphisms are associated with 
      total IgE levels in Korean children with asthma.
PG  - 243-52
LID - 10.3109/02770903.2012.660294 [doi]
AB  - OBJECTIVE: To investigate associations between total serum immunoglobulin E (IgE) 
      levels and single nucleotide polymorphisms (SNPs) from eight candidate genes 
      (IL-4 rs2243250, IL-4Ralpha rs1805010, IL-13 rs20541, IL-13Ralpha1 rs2495636, CD14 
      rs2569190, tumor necrosis factor-alpha (TNF-alpha) rs1800629, cytotoxic T 
      lymphocyte-associated antigen (CTLA4) rs231775, FCER1B rs1441585) in children 
      with asthma and to evaluate gene-gene interactions. METHODS: A total of 669 
      Korean children with asthma (n = 544 atopic n = 125 non-atopic) were included. 
      Asthma phenotypes, total serum IgE levels, and methacholine challenge test 
      results were evaluated. SNPs were genotyped using the polymerase chain 
      reaction-restriction fragment length polymorphisms (PCR-RFLP) method. 
      Multi-factor dimensionality reduction (MDR) was used to analyze gene-gene 
      interactions. RESULTS: The combination of the IL-13, IL-13Ralpha1, and CTLA4 
      polymorphisms was selected through MDR analysis of the data pertaining to 
      children with atopic and non-atopic asthma (accuracy = 0.5459, cross validation 
      consistency (CVC) = 10/10). The IL-4Ralpha, IL-13, IL-13Ralpha1, CD14, and CTLA4 
      polymorphisms were selected as the best model of increased total serum IgE levels 
      in non-atopic and atopic asthma (asthma: accuracy = 0.4726, CVC = 10/10; atopic 
      asthma: accuracy = 0.4573, CVC = 10/10). Both the IL-4Ralpha and the IL-13 
      polymorphisms were correlated with the IgE level. ANOVA analysis revealed that 
      the combinations of the CTLA4 and IL-13, IL-13 and IL-13Ralpha1, IL-4Ralpha and IL-13, 
      and CD14 and IL-13 polymorphisms were all significantly associated with increased 
      total serum IgE levels. CONCLUSIONS: The best model of increased IgE level 
      included the IL-4Ralpha, IL-13, IL-13Ralpha1, CD14, and CTLA4 polymorphisms. Of the 
      various interactions between these polymorphisms, the combinations of the CTLA4 
      and IL-13 polymorphisms and the IL-13 and IL-13Ralpha1 polymorphisms showed 
      synergistic effects in terms of increased total serum IgE levels in the present 
      cohort.
FAU - Choi, Won-Ah
AU  - Choi WA
AD  - Asan Institute for Life Sciences, Seoul, Korea.
FAU - Kang, Mi-Jin
AU  - Kang MJ
FAU - Kim, Young-Joon
AU  - Kim YJ
FAU - Seo, Ju-Hee
AU  - Seo JH
FAU - Kim, Hyung-Young
AU  - Kim HY
FAU - Kwon, Ji-Won
AU  - Kwon JW
FAU - Yu, Jinho
AU  - Yu J
FAU - Park, Seoung-Ju
AU  - Park SJ
FAU - Lee, Yong-Chul
AU  - Lee YC
FAU - Hong, Soo-Jong
AU  - Hong SJ
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20120229
PL  - England
TA  - J Asthma
JT  - The Journal of asthma : official journal of the Association for the Care of 
      Asthma
JID - 8106454
RN  - 0 (CTLA-4 Antigen)
RN  - 0 (CTLA4 protein, human)
RN  - 0 (IL13RA1 protein, human)
RN  - 0 (IL4R protein, human)
RN  - 0 (Interleukin-13)
RN  - 0 (Interleukin-13 Receptor alpha1 Subunit)
RN  - 0 (Interleukin-4 Receptor alpha Subunit)
RN  - 0 (Lipopolysaccharide Receptors)
RN  - 37341-29-0 (Immunoglobulin E)
SB  - IM
MH  - Asian People
MH  - Asthma/*blood/diagnosis/*genetics/immunology
MH  - CTLA-4 Antigen/genetics
MH  - Child
MH  - Child, Preschool
MH  - Female
MH  - Gene Frequency/genetics
MH  - Humans
MH  - Immunoglobulin E/*blood
MH  - Interleukin-13/genetics
MH  - Interleukin-13 Receptor alpha1 Subunit/genetics
MH  - Interleukin-4 Receptor alpha Subunit/genetics
MH  - Lipopolysaccharide Receptors/genetics
MH  - Male
MH  - Polymorphism, Single Nucleotide/*genetics
MH  - Skin Tests
EDAT- 2012/03/02 06:00
MHDA- 2012/05/05 06:00
CRDT- 2012/03/02 06:00
PHST- 2012/03/02 06:00 [entrez]
PHST- 2012/03/02 06:00 [pubmed]
PHST- 2012/05/05 06:00 [medline]
AID - 10.3109/02770903.2012.660294 [doi]
PST - ppublish
SO  - J Asthma. 2012 Apr;49(3):243-52. doi: 10.3109/02770903.2012.660294. Epub 2012 Feb 
      29.