PMID- 22377264
OWN - NLM
STAT- MEDLINE
DCOM- 20120716
LR  - 20171116
IS  - 1873-4200 (Electronic)
IS  - 0301-4622 (Linking)
VI  - 163-164
DP  - 2012 Apr
TI  - Kinetics of aggregation of UV-irradiated glyceraldehyde-3-phosphate dehydrogenase 
      from rabbit skeletal muscle. Effect of agents possessing chaperone-like activity.
PG  - 11-20
LID - 10.1016/j.bpc.2012.02.001 [doi]
AB  - An aggregation test system based on the aggregation of UV-irradiated 
      glyceraldehyde-3-phosphate dehydrogenase (GAPDH) from rabbit skeletal muscle has 
      been proposed. On the basis of the measurements of the enzyme activity and 
      differential scanning calorimetry data a conclusion has been made that UV 
      radiation results in formation of damaged protein molecules with lower 
      thermostability. It was shown that the order of aggregation rate for 
      UV-irradiated GAPDH with respect to the protein was close to 2. This means that 
      such a test system allows detecting the effect of various agents exclusively on 
      the stage of aggregation of unfolded protein molecules. The influence of 
      alpha-crystallin and 2-hydroxypropyl-beta-cyclodextrin (HP-beta-CD) on aggregation of 
      UV-irradiated GAPDH was studied. Despite the fact that HP-beta-CD accelerates 
      thermal aggregation of non-irradiated GAPDH, in the case of aggregation of 
      UV-irradiated GAPDH HP-beta-CD reveals a purely protective effect.
CI  - Copyright (c) 2012 Elsevier B.V. All rights reserved.
FAU - Maloletkina, Olga I
AU  - Maloletkina OI
AD  - Bach Institute of Biochemistry, Russian Academy of Sciences, Moscow, Russia.
FAU - Markossian, Kira A
AU  - Markossian KA
FAU - Chebotareva, Natalia A
AU  - Chebotareva NA
FAU - Asryants, Regina A
AU  - Asryants RA
FAU - Kleymenov, Sergey Yu
AU  - Kleymenov SY
FAU - Poliansky, Nikolay B
AU  - Poliansky NB
FAU - Muranov, Konstantin O
AU  - Muranov KO
FAU - Makeeva, Valentina F
AU  - Makeeva VF
FAU - Kurganov, Boris I
AU  - Kurganov BI
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20120210
PL  - Netherlands
TA  - Biophys Chem
JT  - Biophysical chemistry
JID - 0403171
RN  - 0 (Molecular Chaperones)
RN  - 0 (alpha-Crystallins)
RN  - 0 (beta-Cyclodextrins)
RN  - 1I96OHX6EK (2-Hydroxypropyl-beta-cyclodextrin)
RN  - EC 1.2.1.- (Glyceraldehyde-3-Phosphate Dehydrogenases)
SB  - IM
MH  - 2-Hydroxypropyl-beta-cyclodextrin
MH  - Animals
MH  - Glyceraldehyde-3-Phosphate Dehydrogenases/chemistry/*metabolism
MH  - Kinetics
MH  - Molecular Chaperones/chemistry
MH  - Muscle, Skeletal/*enzymology
MH  - Protein Denaturation
MH  - Protein Stability
MH  - Rabbits
MH  - Temperature
MH  - *Ultraviolet Rays
MH  - alpha-Crystallins/chemistry
MH  - beta-Cyclodextrins/chemistry
EDAT- 2012/03/02 06:00
MHDA- 2012/07/17 06:00
CRDT- 2012/03/02 06:00
PHST- 2011/12/06 00:00 [received]
PHST- 2012/01/30 00:00 [revised]
PHST- 2012/02/04 00:00 [accepted]
PHST- 2012/03/02 06:00 [entrez]
PHST- 2012/03/02 06:00 [pubmed]
PHST- 2012/07/17 06:00 [medline]
AID - S0301-4622(12)00040-3 [pii]
AID - 10.1016/j.bpc.2012.02.001 [doi]
PST - ppublish
SO  - Biophys Chem. 2012 Apr;163-164:11-20. doi: 10.1016/j.bpc.2012.02.001. Epub 2012 
      Feb 10.