PMID- 22377751
OWN - NLM
STAT- MEDLINE
DCOM- 20120703
LR  - 20131121
IS  - 1791-3004 (Electronic)
IS  - 1791-2997 (Linking)
VI  - 5
IP  - 5
DP  - 2012 May
TI  - Calcium oxalate monohydrate crystals stimulate monocyte chemoattractant protein-1 
      and transforming growth factor beta1 expression in human renal epithelial cells.
PG  - 1241-4
LID - 10.3892/mmr.2012.813 [doi]
AB  - Crystal-cell interactions play a key role in the formation of kidney stones. Few 
      studies have referred to the role of monocyte chemoattractant protein-1 (MCP-1) 
      and transforming growth factor beta1 (TGFbeta1) in kidney stone formation. Recently, a 
      genome-wide analysis of genes related to kidney stone formation and eliminiation 
      in mice indicated that MCP-1 and TGFbeta1 are involved in nephrolithiasis. In this 
      study, in order to verify whether MCP-1 and TGFbeta1 are involved in the process of 
      crystal-cell interactions in vitro, we observed the effects of calcium oxalate 
      monohydrate (COM) on MCP-1 and TGFbeta1 expression in cultured HK-2 cells. HK-2 
      cells were treated with different concentrations of COM, and a group of untreated 
      cells served as the control. The expression of MCP-1 and TGFbeta1 was detected by 
      western blot analysis after treatments with different COM concentrations (300, 
      500, 700 and 900 microg/ml) for different times (3, 6, 12 and 24 h). We found that 
      the expression of MCP-1 was upregulated by COM treatment in a dose-dependent 
      manner, and was increased initially at the first 6 h of treatment, then slightly 
      decreased over time. Also, COM treatment resulted in a dose-dependent increase in 
      TGFbeta1 expression, and the expression levels peaked at 12 h. This study 
      demonstrates that COM stimulates the expression of MCP-1 and TGFbeta1 in renal 
      epithelial cells.
FAU - Liu, Zhuo
AU  - Liu Z
AD  - Department of Urology, Tongji Hospital, Tongji Medical College, Huazhong 
      University of Science and Technology, Wuhan, Hubei 430030, PR China.
FAU - Wang, Tao
AU  - Wang T
FAU - Yang, Jun
AU  - Yang J
FAU - Wang, Shaogang
AU  - Wang S
FAU - Yang, Weimin
AU  - Yang W
FAU - Liu, Jihong
AU  - Liu J
FAU - Ye, Zhangqun
AU  - Ye Z
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20120228
PL  - Greece
TA  - Mol Med Rep
JT  - Molecular medicine reports
JID - 101475259
RN  - 0 (CCL2 protein, human)
RN  - 0 (Chemokine CCL2)
RN  - 0 (Transforming Growth Factor beta1)
RN  - 2612HC57YE (Calcium Oxalate)
SB  - IM
MH  - Animals
MH  - Calcium Oxalate/*pharmacology
MH  - Cell Line
MH  - Chemokine CCL2/*biosynthesis
MH  - Dose-Response Relationship, Drug
MH  - Epithelial Cells/*metabolism/pathology
MH  - Humans
MH  - Kidney/*metabolism/pathology
MH  - Mice
MH  - Nephrolithiasis/metabolism/pathology
MH  - Time Factors
MH  - Transforming Growth Factor beta1/*biosynthesis
MH  - Up-Regulation/*drug effects
EDAT- 2012/03/02 06:00
MHDA- 2012/07/04 06:00
CRDT- 2012/03/02 06:00
PHST- 2011/11/24 00:00 [received]
PHST- 2012/02/16 00:00 [accepted]
PHST- 2012/03/02 06:00 [entrez]
PHST- 2012/03/02 06:00 [pubmed]
PHST- 2012/07/04 06:00 [medline]
AID - 10.3892/mmr.2012.813 [doi]
PST - ppublish
SO  - Mol Med Rep. 2012 May;5(5):1241-4. doi: 10.3892/mmr.2012.813. Epub 2012 Feb 28.