PMID- 22379747
OWN - NLM
STAT- MEDLINE
DCOM- 20120405
LR  - 20141120
IS  - 0125-2208 (Print)
IS  - 0125-2208 (Linking)
VI  - 95
IP  - 1
DP  - 2012 Jan
TI  - Human epidermal growth-factor receptor 2 overexpression in gastric carcinoma in 
      Thai patients.
PG  - 88-95
AB  - OBJECTIVE: To determine human epidermal growth-factor receptor 2 (HER2) 
      protein-overexpression frequency and the concordance rate between 
      immunohistochemistry and fluorescence in situ hybridization techniques in gastric 
      carcinoma. MATERIAL AND METHOD: A retrospective analysis of gastric 
      adenocarcinomas obtained from 224 adult patients between January 2000 and 
      December 2008 were performed. The paraffin-embedded tissues were sliced into 
      4-microm-thick sections and analyzed for HER2 protein expression levels by 
      immunohistochemistry (IHC) using an automated slide-staining IHC system. Breast 
      carcinoma tissues were included in every staining batch as positive control. In 
      order to detect and quantify amplification of the HER2, the authors performed 
      fluorescence in situ hybridization (FISH) using PathVysion HER2 DNA Probe Kit. 
      The IHC results were independently recorded by two pathologists using the 
      standard HER2 scoring system for gastric carcinoma. FISH results were interpreted 
      using standard guideline as employed in breast carcinoma. The two-tailed-Fisher's 
      exact test was used to assess the concordance between IHC and FISH results. 
      RESULTS: HER2 protein overexpression level was identified in 9% (20 in 224 cases) 
      of the gastric tumors; 80% of which were well or moderately differentiated and of 
      the intestinal or mixed type. However HER2-overexpressing tumors comprised only 
      16% of the intestinal/mixed-type or well/moderately differentiated tumors. There 
      was no signal obtained from 29 specimens from FISH studies. Thus, the overall 
      results of IHC and FISH methods were concordant in 88% (171 out of 195, p < 
      0.001). CONCLUSION: There is a significant concordance rate between IHC and FISH 
      in gastric carcinoma. The present study is the first HER2 study of such carcinoma 
      in Thai patients.
FAU - Sornmayura, Pattana
AU  - Sornmayura P
AD  - Department of Pathology, Faculty of Medicine, Ramathibodi Hospital, Mahidol 
      University, Bangkok, Thailand. p2517s@yahoo.com
FAU - Rerkamnuaychoke, Budsaba
AU  - Rerkamnuaychoke B
FAU - Jinawath, Artit
AU  - Jinawath A
FAU - Euanorasetr, Chakrapan
AU  - Euanorasetr C
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Thailand
TA  - J Med Assoc Thai
JT  - Journal of the Medical Association of Thailand = Chotmaihet thangphaet
JID - 7507216
RN  - EC 2.7.10.1 (Receptor, ErbB-2)
SB  - IM
MH  - Adenocarcinoma/*metabolism/pathology
MH  - Adolescent
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Female
MH  - Humans
MH  - Immunohistochemistry
MH  - In Situ Hybridization, Fluorescence
MH  - Male
MH  - Middle Aged
MH  - Receptor, ErbB-2/*metabolism
MH  - Retrospective Studies
MH  - Stomach Neoplasms/*metabolism/pathology
MH  - Thailand
EDAT- 2012/03/03 06:00
MHDA- 2012/04/06 06:00
CRDT- 2012/03/03 06:00
PHST- 2012/03/03 06:00 [entrez]
PHST- 2012/03/03 06:00 [pubmed]
PHST- 2012/04/06 06:00 [medline]
PST - ppublish
SO  - J Med Assoc Thai. 2012 Jan;95(1):88-95.