PMID- 22381256
OWN - NLM
STAT- MEDLINE
DCOM- 20120824
LR  - 20240109
IS  - 1873-6351 (Electronic)
IS  - 0278-6915 (Linking)
VI  - 50
IP  - 5
DP  - 2012 May
TI  - Effects of [6]-shogaol on cholinergic signaling in HT22 cells following neuronal 
      damage induced by hydrogen peroxide.
PG  - 1454-9
LID - 10.1016/j.fct.2012.02.014 [doi]
AB  - Cholinergic neurons play a major role in memory and attention. The dysfunction 
      and death of these neurons, especially in the hippocampus, are thought to 
      contribute to the pathophysiology of memory deficits associated with Alzheimer's 
      disease (AD). Therefore, studying the cholinergic properties and cell survival 
      may help in treating this disease. We investigated the possible effects of 
      [6]-shogaol on cholinergic signaling in HT22 hippocampal neuronal cells. HT22 
      cells express essential cholinergic markers, including choline acetyltransferase 
      (ChAT) and choline transporter (ChTp). HT22 cells treated with H(2)O(2) for 3h 
      showed an increase in ROS production (35%). These features were partly recovered 
      by [6]-shogaol. Treating H(2)O(2)-treated HT22 cells with [6]-shogaol markedly 
      increased the expression of ChAT and ChTp, an effect similar to that of 
      brain-derived neurotrophic factor (BDNF). Furthermore, K-252a, an inhibitor of 
      the BDNF receptor Trk B, attenuated the effects of both [6]-shogaol and BDNF. 
      These data suggest that [6]-shogaol protects neurons by increasing ChAT and ChTp 
      expression through a BDNF increase and thus may be useful for treating 
      neurodegenerative diseases.
CI  - Copyright (c) 2012 Elsevier Ltd. All rights reserved.
FAU - Shim, Sehwan
AU  - Shim S
AD  - Department of Laboratory Animal Medicine, College of Veterinary Medicine, Chonbuk 
      National University, Jeonju 561 756, Republic of Korea.
FAU - Kwon, Jungkee
AU  - Kwon J
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20120222
PL  - England
TA  - Food Chem Toxicol
JT  - Food and chemical toxicology : an international journal published for the British 
      Industrial Biological Research Association
JID - 8207483
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Catechols)
RN  - 0 (Reactive Oxygen Species)
RN  - 0 (Receptors, Cholinergic)
RN  - 83DNB5FIRF (shogaol)
RN  - BBX060AN9V (Hydrogen Peroxide)
SB  - IM
MH  - Brain-Derived Neurotrophic Factor/pharmacology
MH  - Catechols/*pharmacology
MH  - Cell Line
MH  - Humans
MH  - Hydrogen Peroxide/*toxicity
MH  - Neurons/*drug effects
MH  - Reactive Oxygen Species/metabolism
MH  - Receptors, Cholinergic/*metabolism
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Signal Transduction/*drug effects
EDAT- 2012/03/03 06:00
MHDA- 2012/08/25 06:00
CRDT- 2012/03/03 06:00
PHST- 2011/11/03 00:00 [received]
PHST- 2012/02/08 00:00 [revised]
PHST- 2012/02/10 00:00 [accepted]
PHST- 2012/03/03 06:00 [entrez]
PHST- 2012/03/03 06:00 [pubmed]
PHST- 2012/08/25 06:00 [medline]
AID - S0278-6915(12)00097-X [pii]
AID - 10.1016/j.fct.2012.02.014 [doi]
PST - ppublish
SO  - Food Chem Toxicol. 2012 May;50(5):1454-9. doi: 10.1016/j.fct.2012.02.014. Epub 
      2012 Feb 22.