PMID- 22382092
OWN - NLM
STAT- MEDLINE
DCOM- 20120717
LR  - 20120312
IS  - 1873-376X (Electronic)
IS  - 1570-0232 (Linking)
VI  - 889-890
DP  - 2012 Mar 15
TI  - Molecular imprinting based composite cryogel membranes for purification of 
      anti-hepatitis B surface antibody by fast protein liquid chromatography.
PG  - 95-102
LID - 10.1016/j.jchromb.2012.02.001 [doi]
AB  - In the present study, we have focused our attention to prepare molecular 
      imprinted composite cryogel membranes for purification of hepatitis B surface 
      antibody (anti-HBs) by fast protein liquid chromatography. Before the preparation 
      of the molecular imprinted composite cryogel membranes (MI-CMs) by free radical 
      polymerization at sub-zero temperature, we have synthesized and characterized the 
      anti-HBs imprinted particles. Then, the cryogel membranes (CMs) were 
      characterized by swelling test, scanning electron microscopy and Fourier 
      transform infrared spectroscopy. Prior to chromatographic purification studies, 
      the effective parameters on the anti-HBs adsorption process were evaluated by 
      investigating the dependency of the adsorption capacity on flow-rate, anti-HBs 
      concentration, contact time and ionic strength. The maximum anti-HBs adsorption 
      capacity was calculated as 701.4 mIU/g CM. The selectivity of the MI-CMs was 
      shown by competitive adsorption of anti-HBs, total anti-hepatitis A antibody 
      (anti-HAV) and total immunoglobulin E (IgE) adsorption studies. The MI-CMs have 
      relative selectivity coefficients as 5.45 for anti-HBs/total anti-HAV and 9.05 
      for anti-HBs/total IgE, respectively. The phosphate buffer solution (pH 7.4) 
      containing 1.0M NaCl was used for elution, almost completely, of adsorbed 
      anti-HBs molecules. The MI-CMs could be used many times without any significant 
      decrease in the adsorption capacity. The chromatographic purification 
      performances of the MI-CMs were also investigated. The chromatographic parameters 
      such as capacity and separation factors, the theoretical plate number and 
      resolution of the MI-CMs were calculated as 5.48, 6.02, 1153.9, and 1.72 for 
      anti-HBs molecules, respectively. As a conclusion, we can say that the MI-CMs 
      could be used for specific purification of anti-HBs from anti-HBs positive human 
      plasma.
CI  - Copyright A(c) 2012 Elsevier B.V. All rights reserved.
FAU - Asliyuce, Sevgi
AU  - Asliyuce S
AD  - Hacettepe University, Department of Chemistry, Biochemistry Division, Ankara, 
      Turkey.
FAU - Uzun, Lokman
AU  - Uzun L
FAU - Rad, Abbas Yousefi
AU  - Rad AY
FAU - Unal, Serhat
AU  - Unal S
FAU - Say, Ridvan
AU  - Say R
FAU - Denizli, Adil
AU  - Denizli A
LA  - eng
PT  - Journal Article
DEP - 20120209
PL  - Netherlands
TA  - J Chromatogr B Analyt Technol Biomed Life Sci
JT  - Journal of chromatography. B, Analytical technologies in the biomedical and life 
      sciences
JID - 101139554
RN  - 0 (Cryogels)
RN  - 0 (Hepatitis B Antibodies)
RN  - 0 (Hepatitis B Surface Antigens)
RN  - 0 (Membranes, Artificial)
SB  - IM
MH  - Adsorption
MH  - Chromatography, Affinity/instrumentation/*methods
MH  - Cryogels/*chemistry
MH  - Equipment Reuse
MH  - Hepatitis B/immunology
MH  - Hepatitis B Antibodies/blood/immunology/*isolation & purification
MH  - Hepatitis B Surface Antigens/*immunology
MH  - Humans
MH  - Membranes, Artificial
MH  - Microscopy, Electron, Scanning
MH  - Molecular Imprinting/instrumentation/*methods
MH  - Osmolar Concentration
MH  - Spectroscopy, Fourier Transform Infrared
EDAT- 2012/03/03 06:00
MHDA- 2012/07/18 06:00
CRDT- 2012/03/03 06:00
PHST- 2011/10/07 00:00 [received]
PHST- 2012/01/10 00:00 [revised]
PHST- 2012/02/03 00:00 [accepted]
PHST- 2012/03/03 06:00 [entrez]
PHST- 2012/03/03 06:00 [pubmed]
PHST- 2012/07/18 06:00 [medline]
AID - S1570-0232(12)00076-1 [pii]
AID - 10.1016/j.jchromb.2012.02.001 [doi]
PST - ppublish
SO  - J Chromatogr B Analyt Technol Biomed Life Sci. 2012 Mar 15;889-890:95-102. doi: 
      10.1016/j.jchromb.2012.02.001. Epub 2012 Feb 9.