PMID- 22382292 OWN - NLM STAT- MEDLINE DCOM- 20120629 LR - 20120316 IS - 1873-2976 (Electronic) IS - 0960-8524 (Linking) VI - 110 DP - 2012 Apr TI - Enhanced phenol degradation in coking wastewater by immobilized laccase on magnetic mesoporous silica nanoparticles in a magnetically stabilized fluidized bed. PG - 120-4 LID - 10.1016/j.biortech.2012.01.184 [doi] AB - The immobilized laccase on magnetic mesoporous silica nanoparticles has been developed for efficient phenol degradation. The degradation rate of phenol by the immobilized laccase was 2-fold higher than that of the free laccase, and the immobilized laccase retained 71.3% of its initial degradation ability after 10 successive batch treatments of coking wastewater. The phenol degradation in the coking wastewater was enhanced in a continuous treatment process by the immobilized laccase in a magnetically stabilized fluidized bed (MSFB) because of good mixing and mass transfer. The degradation rate of phenol maintained more than 99% at a flow rate of less than 450mLh(-1) and decreased slowly to 91.5% after 40h of the continuous operation in the MSFB. The present work indicated that the immobilized laccase on magnetic mesoporous supports together with the MSFB provided a promising avenue for the continuous enzymatic degradation of phenolic compounds in industrial wastewater. CI - Copyright (c) 2012 Elsevier Ltd. All rights reserved. FAU - Wang, Feng AU - Wang F AD - National Key Laboratory of Biochemical Engineering & Laboratory of Separation Science and Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, PR China. FAU - Hu, Yiru AU - Hu Y FAU - Guo, Chen AU - Guo C FAU - Huang, Wei AU - Huang W FAU - Liu, Chun-Zhao AU - Liu CZ LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20120211 PL - England TA - Bioresour Technol JT - Bioresource technology JID - 9889523 RN - 0 (Enzymes, Immobilized) RN - 0 (Phenols) RN - 0 (Water Pollutants, Chemical) RN - 7631-86-9 (Silicon Dioxide) RN - EC 1.10.3.2 (Laccase) SB - IM MH - Enzymes, Immobilized/*chemistry MH - Laccase/*chemistry MH - *Magnetics MH - *Nanoparticles MH - Phenols/*chemistry MH - Silicon Dioxide/*chemistry MH - Water Pollutants, Chemical/*chemistry EDAT- 2012/03/03 06:00 MHDA- 2012/06/30 06:00 CRDT- 2012/03/03 06:00 PHST- 2011/11/25 00:00 [received] PHST- 2012/01/16 00:00 [revised] PHST- 2012/01/19 00:00 [accepted] PHST- 2012/03/03 06:00 [entrez] PHST- 2012/03/03 06:00 [pubmed] PHST- 2012/06/30 06:00 [medline] AID - S0960-8524(12)00255-6 [pii] AID - 10.1016/j.biortech.2012.01.184 [doi] PST - ppublish SO - Bioresour Technol. 2012 Apr;110:120-4. doi: 10.1016/j.biortech.2012.01.184. Epub 2012 Feb 11.