PMID- 22384347
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20120910
LR  - 20211021
IS  - 2160-1836 (Electronic)
IS  - 2160-1836 (Linking)
VI  - 1
IP  - 5
DP  - 2011 Oct
TI  - SAD-3, a Putative Helicase Required for Meiotic Silencing by Unpaired DNA, 
      Interacts with Other Components of the Silencing Machinery.
PG  - 369-76
LID - 10.1534/g3.111.000570 [doi]
AB  - In Neurospora crassa, genes lacking a pairing partner during meiosis are 
      suppressed by a process known as meiotic silencing by unpaired DNA (MSUD). To 
      identify novel MSUD components, we have developed a high-throughput 
      reverse-genetic screen for use with the N. crassa knockout library. Here we 
      describe the screening method and the characterization of a gene (sad-3) 
      subsequently discovered. SAD-3 is a putative helicase required for MSUD and 
      sexual spore production. It exists in a complex with other known MSUD proteins in 
      the perinuclear region, a center for meiotic silencing activity. Orthologs of 
      SAD-3 include Schizosaccharomyces pombe Hrr1, a helicase required for 
      RNAi-induced heterochromatin formation. Both SAD-3 and Hrr1 interact with an 
      RNA-directed RNA polymerase and an Argonaute, suggesting that certain aspects of 
      silencing complex formation may be conserved between the two fungal species.
FAU - Hammond, Thomas M
AU  - Hammond TM
FAU - Xiao, Hua
AU  - Xiao H
FAU - Boone, Erin C
AU  - Boone EC
FAU - Perdue, Tony D
AU  - Perdue TD
FAU - Pukkila, Patricia J
AU  - Pukkila PJ
FAU - Shiu, Patrick K T
AU  - Shiu PK
LA  - eng
PT  - Journal Article
DEP - 20111001
PL  - England
TA  - G3 (Bethesda)
JT  - G3 (Bethesda, Md.)
JID - 101566598
PMC - PMC3276149
OTO - NOTNLM
OT  - MSUD
OT  - RNA interference
OT  - epigenetics
OT  - meiosis
EDAT- 2012/03/03 06:00
MHDA- 2012/03/03 06:01
PMCR- 2011/10/01
CRDT- 2012/03/03 06:00
PHST- 2011/06/09 00:00 [received]
PHST- 2011/08/16 00:00 [accepted]
PHST- 2012/03/03 06:00 [entrez]
PHST- 2012/03/03 06:00 [pubmed]
PHST- 2012/03/03 06:01 [medline]
PHST- 2011/10/01 00:00 [pmc-release]
AID - GGG_000570 [pii]
AID - 10.1534/g3.111.000570 [doi]
PST - ppublish
SO  - G3 (Bethesda). 2011 Oct;1(5):369-76. doi: 10.1534/g3.111.000570. Epub 2011 Oct 1.