PMID- 22384559
OWN - NLM
STAT- MEDLINE
DCOM- 20131220
LR  - 20161018
IS  - 1003-5370 (Print)
IS  - 1003-5370 (Linking)
VI  - 31
IP  - 12
DP  - 2011 Dec
TI  - [Langohuangping granule down-regulated monocyte chemoattractant protein-1 via 
      suppressing nuclear factor kappa B signaling pathway in BXSB lupus nephritis 
      mice].
PG  - 1685-9
AB  - OBJECTIVE: To study whether Langchuangping granule (LG) could exert its renal 
      protection by down-regulating monocyte chemoattractant protein-1 (MCP-1) via 
      suppressing nuclear factor kappa B (NF-kappaB) signaling pathway in BXSB lupus 
      nephritis (LN) mice. Methods Eighteen male 11-week-old BXSB LN mice were randomly 
      divided into three groups, i.e., the model group, the hormone group, and the 
      Chinese medicine group, 6 in each. They were administered by gastrogavage with 
      normal saline, methylprednisolone, and LG, respectively. Another six C57BL/6 male 
      mice of the same age was taken as the normal control group, which was 
      administered with normal saline by gastrogavage. All mice were treated once 
      daily, for 4 successive weeks. The 24-h urine protein was determined. The mRNA 
      and protein expressions of MCP-1 in the renal tissue were detected using RT-PCR 
      and Western blot. The expression of NF-kappaB p65 in the renal tissue was 
      detected using immunohistochemical assay. Activity index (AI) of the renal tissue 
      was counted using PAS stain. The content of ds-DNA antibody was detected using 
      ELISA. The correlations of the aforesaid indices were analyzed. RESULTS: The 24-h 
      urine protein level, serum ds-DNA antibody content, protein and mRNA expressions 
      of MCP-1, NF-kappaB p65 expression level, and AI count were obviously higher in 
      the model group than in the normal control group (P < 0.01). The aforesaid 
      indices all obviously decreased after medication in the Chinese medicine group 
      and the hormone group (P < 0.05). MCP-1 protein expression level was positively 
      correlated with MCP-1 mRNA, NF-kappaB p65, AI, 24-h urine protein, and ds-DNA 
      antibody of all LN mice (r= 0.984, 0.936, 0.887, 0.698, 0.679, all P < 0.01). 
      CONCLUSIONS: LG possibly played renal protection by down-regulating 
      NF-kappaB-mediated MCP-1 expression levels. MCP-1 played important roles in the 
      occurrence and development of LN, being one of ideal targets for LN treatment.
FAU - Li, Gui-ying
AU  - Li GY
AD  - Department of Nephrology, The Affiliated Hospital of Hebei Engineering 
      University, Hebei. fflgy@126.com
FAU - Lu, Xin-qing
AU  - Lu XQ
FAU - Liu, Shu-xia
AU  - Liu SX
LA  - chi
PT  - English Abstract
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - China
TA  - Zhongguo Zhong Xi Yi Jie He Za Zhi
JT  - Zhongguo Zhong xi yi jie he za zhi Zhongguo Zhongxiyi jiehe zazhi = Chinese 
      journal of integrated traditional and Western medicine
JID - 9211576
RN  - 0 (Chemokine CCL2)
RN  - 0 (Drugs, Chinese Herbal)
RN  - 0 (NF-kappa B)
SB  - IM
MH  - Animals
MH  - Chemokine CCL2/*metabolism
MH  - Disease Models, Animal
MH  - Down-Regulation
MH  - Drugs, Chinese Herbal/*pharmacology
MH  - Kidney/*drug effects/metabolism
MH  - Lupus Nephritis/*metabolism
MH  - Male
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - NF-kappa B/*metabolism
MH  - Signal Transduction/drug effects
EDAT- 2012/03/06 06:00
MHDA- 2013/12/21 06:00
CRDT- 2012/03/06 06:00
PHST- 2012/03/06 06:00 [entrez]
PHST- 2012/03/06 06:00 [pubmed]
PHST- 2013/12/21 06:00 [medline]
PST - ppublish
SO  - Zhongguo Zhong Xi Yi Jie He Za Zhi. 2011 Dec;31(12):1685-9.