PMID- 22386775 OWN - NLM STAT- MEDLINE DCOM- 20120928 LR - 20120309 IS - 1873-2682 (Electronic) IS - 1011-1344 (Linking) VI - 109 DP - 2012 Apr 2 TI - Photophysics of DNA staining dye Propidium Iodide encapsulated in bio-mimetic micelle and genomic fish sperm DNA. PG - 58-67 LID - 10.1016/j.jphotobiol.2012.02.001 [doi] AB - Photophysical processes in Propidium Iodide (PI), the well known DNA staining dye, have been exploited in homogeneous as well as heterogeneous medium by steady state and time resolved spectroscopy. The DNA staining dye PI exhibits intermolecular proton transfer reaction in aqueous and hydrogen-bonding acceptor solvents due to the formation of quinonoid structure which is acidic in nature. Time resolved emission spectroscopy also predicts the hydrogen bond donor ability of PI. The target dye interacts only with anionic surfactant sodium dodecylsulphate but not with cationic surfactant cetyltrimethylammonium bromide and neutral surfactant Triton X-100 and this interaction is found to be electrostatic in nature. We have further scrutinized the mode of binding of PI with fish sperm DNA. The effect of addition of urea, fluorescence quenching phenomenon, CD measurements reveal that the probe binds to DNA through intercalative style. CI - Copyright A(c) 2012 Elsevier B.V. All rights reserved. FAU - Samanta, Anuva AU - Samanta A AD - Department of Chemistry, University of Calcutta, 92, A.P.C. Road, Kolkata 700 009, India. FAU - Paul, Bijan Kumar AU - Paul BK FAU - Guchhait, Nikhil AU - Guchhait N LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20120215 PL - Switzerland TA - J Photochem Photobiol B JT - Journal of photochemistry and photobiology. B, Biology JID - 8804966 RN - 0 (Capsules) RN - 0 (Coloring Agents) RN - 0 (Micelles) RN - 0 (Protons) RN - 36015-30-2 (Propidium) RN - 9007-49-2 (DNA) SB - IM MH - Absorption MH - Animals MH - Biomimetic Materials/*chemistry MH - Capsules MH - Circular Dichroism MH - Coloring Agents/chemistry MH - DNA/*chemistry MH - *Fishes MH - Genome/*genetics MH - Male MH - *Micelles MH - Propidium/*chemistry MH - Protons MH - Spectrometry, Fluorescence MH - *Spermatozoa EDAT- 2012/03/06 06:00 MHDA- 2012/09/29 06:00 CRDT- 2012/03/06 06:00 PHST- 2012/01/04 00:00 [received] PHST- 2012/02/03 00:00 [revised] PHST- 2012/02/06 00:00 [accepted] PHST- 2012/03/06 06:00 [entrez] PHST- 2012/03/06 06:00 [pubmed] PHST- 2012/09/29 06:00 [medline] AID - S1011-1344(12)00025-5 [pii] AID - 10.1016/j.jphotobiol.2012.02.001 [doi] PST - ppublish SO - J Photochem Photobiol B. 2012 Apr 2;109:58-67. doi: 10.1016/j.jphotobiol.2012.02.001. Epub 2012 Feb 15.