PMID- 22389734
OWN - NLM
STAT- MEDLINE
DCOM- 20120622
LR  - 20211021
IS  - 1935-2735 (Electronic)
IS  - 1935-2727 (Print)
IS  - 1935-2727 (Linking)
VI  - 6
IP  - 2
DP  - 2012
TI  - Therapeutic DNA vaccine encoding peptide P10 against experimental 
      paracoccidioidomycosis.
PG  - e1519
LID - 10.1371/journal.pntd.0001519 [doi]
LID - e1519
AB  - Paracoccidioidomycosis (PCM), caused by Paracoccidioides brasiliensis, is the 
      most prevalent invasive fungal disease in South America. Systemic mycoses are the 
      10th most common cause of death among infectious diseases in Brazil and PCM is 
      responsible for more than 50% of deaths due to fungal infections. PCM is 
      typically treated with sulfonamides, amphotericin B or azoles, although complete 
      eradication of the fungus may not occur and relapsing disease is frequently 
      reported. A 15-mer peptide from the major diagnostic antigen gp43, named P10, can 
      induce a strong T-CD4+ helper-1 immune response in mice. The TEPITOPE algorithm 
      and experimental data have confirmed that most HLA-DR molecules can present P10, 
      which suggests that P10 is a candidate antigen for a PCM vaccine. In the current 
      work, the therapeutic efficacy of plasmid immunization with P10 and/or IL-12 
      inserts was tested in murine models of PCM. When given prior to or after 
      infection with P. brasiliensis virulent Pb 18 isolate, plasmid-vaccination with 
      P10 and/or IL-12 inserts successfully reduced the fungal burden in lungs of 
      infected mice. In fact, intramuscular administration of a combination of plasmids 
      expressing P10 and IL-12 given weekly for one month, followed by single 
      injections every month for 3 months restored normal lung architecture and 
      eradicated the fungus in mice that were infected one month prior to treatment. 
      The data indicate that immunization with these plasmids is a powerful procedure 
      for prevention and treatment of experimental PCM, with the perspective of being 
      also effective in human patients.
FAU - Rittner, Glauce M G
AU  - Rittner GM
AD  - Institute of Biomedical Sciences, Department of Microbiology, University of Sao 
      Paulo, Sao Paulo, Sao Paulo, Brazil.
FAU - Munoz, Julian E
AU  - Munoz JE
FAU - Marques, Alexandre F
AU  - Marques AF
FAU - Nosanchuk, Joshua D
AU  - Nosanchuk JD
FAU - Taborda, Carlos P
AU  - Taborda CP
FAU - Travassos, Luiz R
AU  - Travassos LR
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20120228
PL  - United States
TA  - PLoS Negl Trop Dis
JT  - PLoS neglected tropical diseases
JID - 101291488
RN  - 0 (Antigens, Fungal)
RN  - 0 (Fungal Vaccines)
RN  - 0 (Glycoproteins)
RN  - 0 (P10 peptide, Paracoccidioides brasiliensis)
RN  - 0 (Peptide Fragments)
RN  - 0 (Vaccines, DNA)
SB  - IM
MH  - Animals
MH  - Antigens, Fungal/*immunology
MH  - Colony Count, Microbial
MH  - Disease Models, Animal
MH  - Fungal Vaccines/administration & dosage/*immunology
MH  - Genetic Vectors
MH  - Glycoproteins/*immunology
MH  - Immunotherapy/*methods
MH  - Injections, Intramuscular
MH  - Lung/microbiology/pathology
MH  - Male
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Paracoccidioides/*immunology
MH  - Paracoccidioidomycosis/immunology/*therapy
MH  - Peptide Fragments/*immunology
MH  - Plasmids
MH  - Vaccines, DNA/administration & dosage/*immunology
PMC - PMC3289603
COIS- The authors have declared that no competing interests exist.
EDAT- 2012/03/06 06:00
MHDA- 2012/06/23 06:00
PMCR- 2012/02/28
CRDT- 2012/03/06 06:00
PHST- 2011/04/08 00:00 [received]
PHST- 2011/12/23 00:00 [accepted]
PHST- 2012/03/06 06:00 [entrez]
PHST- 2012/03/06 06:00 [pubmed]
PHST- 2012/06/23 06:00 [medline]
PHST- 2012/02/28 00:00 [pmc-release]
AID - PNTD-D-11-00333 [pii]
AID - 10.1371/journal.pntd.0001519 [doi]
PST - ppublish
SO  - PLoS Negl Trop Dis. 2012;6(2):e1519. doi: 10.1371/journal.pntd.0001519. Epub 2012 
      Feb 28.