PMID- 22390843
OWN - NLM
STAT- MEDLINE
DCOM- 20130416
LR  - 20120416
IS  - 1878-3511 (Electronic)
IS  - 1201-9712 (Linking)
VI  - 16
IP  - 5
DP  - 2012 May
TI  - High frequency of Helicobacter pylori in the esophageal mucosa of dyspeptic 
      patients and its possible association with histopathological alterations.
PG  - e364-70
LID - 10.1016/j.ijid.2012.01.007 [doi]
AB  - BACKGROUND: Helicobacter pylori gastric colonization is known to be high in 
      symptomatic subjects. However, only a few reports on the presence of H. pylori in 
      the esophageal mucosa have been published. The aim of this study was to assess 
      the frequency of H. pylori in the esophagus of dyspeptic patients and its 
      association with histopathology. METHODS: The presence of H. pylori in the 
      gastroesophageal mucosa was detected by fluorescence in situ hybridization (FISH) 
      and PCR analysis of DNA extracted from gastric and esophageal biopsies of 82 
      symptomatic patients, using genus- and species-specific PCR primers. Alterations 
      in the gastroesophageal mucosa were assessed by conventional histological 
      techniques. RESULTS: H. pylori in the stomach was detected by PCR and FISH, 
      respectively, in 61% (n=43) and 90% (n=63) of dyspeptic patients, and in the 
      esophagus in 70% (n=44) and 73% (n=46). The prevalence of cagA-positive strains 
      by PCR varied from 50% (n=35) in the gastric mucosa to 65% (n=41) in the 
      esophageal mucosa. By combining the results of both methods, H. pylori was 
      present in the gastroesophageal mucosa in 86% (n=68) of patients. The association 
      of the presence of bacteria, including H. pylori, in the esophageal mucosa with 
      histopathological alterations was statistically significant between 
      microabscesses and bacteria (r=0.656, p<0.0001) and PCR detection and 
      pseudogoblet cells (r=0.25, p<0.047). CONCLUSIONS: This is the first report of 
      the occurrence of H. pylori in the esophageal mucosa from dyspeptic Venezuelan 
      patients. These results demonstrate the high prevalence of H. pylori in the 
      esophagus, and its presence was correlated with signs of inflammation.
CI  - Copyright (c) 2012 International Society for Infectious Diseases. Published by 
      Elsevier Ltd. All rights reserved.
FAU - Contreras, Monica
AU  - Contreras M
AD  - Laboratorio de Fisiologia Gastrointestinal, Centro de Biofisica y Bioquimica, 
      Instituto Venezolano de Investigaciones Cientificas, Km. 11 Carretera 
      Panamericana, Miranda, Venezuela. mocontre@ivic.gob.ve
FAU - Salazar, Victor
AU  - Salazar V
FAU - Garcia-Amado, Maria Alexandra
AU  - Garcia-Amado MA
FAU - Reyes, Nelson
AU  - Reyes N
FAU - Aparcero, Miguel
AU  - Aparcero M
FAU - Silva, Olga
AU  - Silva O
FAU - Castro, Denny
AU  - Castro D
FAU - Romero, Roberto
AU  - Romero R
FAU - Gueneau, Pulcherie
AU  - Gueneau P
FAU - Michelangeli, Fabian
AU  - Michelangeli F
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20120303
PL  - Canada
TA  - Int J Infect Dis
JT  - International journal of infectious diseases : IJID : official publication of the 
      International Society for Infectious Diseases
JID - 9610933
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Aged
MH  - Dyspepsia/diagnosis/epidemiology/*microbiology
MH  - Esophagus/*microbiology/pathology
MH  - Female
MH  - Helicobacter Infections/diagnosis/epidemiology/*microbiology
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Male
MH  - Middle Aged
MH  - Molecular Diagnostic Techniques
MH  - Mucous Membrane/microbiology/pathology
MH  - Polymerase Chain Reaction
MH  - Prevalence
MH  - Stomach/microbiology/pathology
MH  - Young Adult
EDAT- 2012/03/07 06:00
MHDA- 2013/04/17 06:00
CRDT- 2012/03/07 06:00
PHST- 2011/08/03 00:00 [received]
PHST- 2011/11/25 00:00 [revised]
PHST- 2012/01/10 00:00 [accepted]
PHST- 2012/03/07 06:00 [entrez]
PHST- 2012/03/07 06:00 [pubmed]
PHST- 2013/04/17 06:00 [medline]
AID - S1201-9712(12)00040-9 [pii]
AID - 10.1016/j.ijid.2012.01.007 [doi]
PST - ppublish
SO  - Int J Infect Dis. 2012 May;16(5):e364-70. doi: 10.1016/j.ijid.2012.01.007. Epub 
      2012 Mar 3.