PMID- 22391182
OWN - NLM
STAT- MEDLINE
DCOM- 20121220
LR  - 20161018
IS  - 1009-2137 (Print)
IS  - 1009-2137 (Linking)
VI  - 20
IP  - 1
DP  - 2012 Feb
TI  - [Bactericidal permeability increasing protein inhibits 
      lipopolysaccharide-mediated platelet activation in vitro].
PG  - 129-32
AB  - This study was purposed to investigate the inhibitory effect of bactericidal 
      permeability-increasing protein (BPI) on lipopolysaccharide (LPS)-mediated 
      activation of platelets. Venous blood samples were obtained from 10 healthy 
      volunteers and were prepared into platelet-rich plasma (PRP, 1 x 10(8)/ml). 
      Experiments were divided into four groups: normal platelet group (untreated 
      group); LPS group, BPI group and BPI+LPS group. PRP were stimulated by LPS (10 
      microg/ml) in the presence and absence of BPI (100 microg/ml) or BPI alone. Then 
      platelets were harvested and determined for Toll-like receptor-4 (TLR-4) with 
      flow cytometry (FCM), the supernatant was used for detection of cytokines 
      including tumor necrosis factor-alpha (TNF-alpha) and interleukin-6 (IL-6) with 
      enzyme-linked immunosorbent assay (ELISA). The results showed that as compared 
      with normal platelet group, TLR-4 expression on platelets was significantly 
      increased under LPS stimulation (P < 0.001); the levels of TNF-alpha and IL-6 in the 
      supernatant were also remarkably elevated (P < 0.001). However, either TLR-4 
      expression or the cytokine levels significantly decreased in the presence of BPI 
      when platelets underwent LPS-challenge (P < 0.05), but still were higher than 
      that in normal platelet group. Stimulating the platelets with BPI alone could not 
      enhance the TLR-4 expression and cytokine levels. It is concluded that BPI has 
      the ability to inhibit the LPS-induced platelet activation.
FAU - Luo, Xian-Ming
AU  - Luo XM
AD  - Department of Hematology, Sun Yat-Sen University, Guangdong Province, China.
FAU - Yang, Qiu-Hong
AU  - Yang QH
FAU - Wei, Jing
AU  - Wei J
FAU - Ma, Li-Ping
AU  - Ma LP
LA  - chi
PT  - English Abstract
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - China
TA  - Zhongguo Shi Yan Xue Ye Xue Za Zhi
JT  - Zhongguo shi yan xue ye xue za zhi
JID - 101084424
RN  - 0 (Antimicrobial Cationic Peptides)
RN  - 0 (Blood Proteins)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (TLR4 protein, human)
RN  - 0 (Toll-Like Receptor 4)
RN  - 0 (bactericidal permeability increasing protein)
SB  - IM
MH  - Antimicrobial Cationic Peptides/*pharmacology
MH  - Blood Proteins/*pharmacology
MH  - Humans
MH  - Inflammation
MH  - Lipopolysaccharides/adverse effects
MH  - Platelet Activation/*drug effects
MH  - Platelet-Rich Plasma/*metabolism
MH  - Toll-Like Receptor 4/metabolism
EDAT- 2012/03/07 06:00
MHDA- 2012/12/21 06:00
CRDT- 2012/03/07 06:00
PHST- 2012/03/07 06:00 [entrez]
PHST- 2012/03/07 06:00 [pubmed]
PHST- 2012/12/21 06:00 [medline]
AID - 1009-2137(2012)01-0129-04 [pii]
PST - ppublish
SO  - Zhongguo Shi Yan Xue Ye Xue Za Zhi. 2012 Feb;20(1):129-32.