PMID- 22391529
OWN - NLM
STAT- MEDLINE
DCOM- 20120913
LR  - 20211021
IS  - 1098-6596 (Electronic)
IS  - 0066-4804 (Print)
IS  - 0066-4804 (Linking)
VI  - 56
IP  - 6
DP  - 2012 Jun
TI  - L-selectin and P-selectin are novel biomarkers of cervicovaginal inflammation for 
      preclinical mucosal safety assessment of anti-HIV-1 microbicide.
PG  - 3121-32
LID - 10.1128/AAC.05950-11 [doi]
AB  - A major obstacle thwarting preclinical development of microbicides is the lack of 
      a validated biomarker of cervicovaginal inflammation. Therefore, the present 
      study aims to identify novel noninvasive soluble markers in a murine model for 
      assessment of microbicide mucosal safety. By performing cytokine antibody array 
      analysis, we identified two adhesion molecules, L-selectin and P-selectin, which 
      significantly increased when mucosal inflammation was triggered by nonoxynol-9 
      (N9), an anti-HIV-1 microbicide candidate that failed clinical trials, in a 
      refined murine model of agent-induced cervicovaginal inflammation. We found that 
      patterns of detection of L-selectin and P-selectin were obviously different from 
      those of the two previously defined biomarkers of cervicovaginal inflammation, 
      monocyte chemotactic protein 1 (MCP-1) and interleukin 6 (IL-6). The levels of 
      these two soluble selectins correlated better than those of MCP-1 and IL-6 with 
      the duration and severity of mucosal inflammation triggered by N9 and two 
      approved proinflammatory compounds, benzalkonium chloride (BZK) and sodium 
      dodecyl sulfate (SDS), but not by two nonproinflammatory compounds, carboxymethyl 
      celluose (CMC; microbicide excipients) and tenofovir (TFV; microbicide 
      candidate). These data indicated that L-selectin and P-selectin can serve as 
      additional novel cervicovaginal inflammation biomarkers for preclinical mucosal 
      safety evaluation of candidate microbicides for the prevention of infection with 
      HIV and other sexually transmitted pathogens.
FAU - Zhong, Maohua
AU  - Zhong M
AD  - Mucosal Immunity Research Group, State Key Laboratory of Virology, Wuhan 
      Institute of Virology, Chinese Academy of Sciences, Wuhan, China.
FAU - He, Benxia
AU  - He B
FAU - Yang, Jingyi
AU  - Yang J
FAU - Bao, Rong
AU  - Bao R
FAU - Zhang, Yan
AU  - Zhang Y
FAU - Zhou, Dihan
AU  - Zhou D
FAU - Chen, Yaoqing
AU  - Chen Y
FAU - Li, Liangzhu
AU  - Li L
FAU - Han, Chen
AU  - Han C
FAU - Yang, Yi
AU  - Yang Y
FAU - Sun, Ying
AU  - Sun Y
FAU - Cao, Yuan
AU  - Cao Y
FAU - Li, Yaoming
AU  - Li Y
FAU - Shi, Wei
AU  - Shi W
FAU - Jiang, Shibo
AU  - Jiang S
FAU - Zhang, Xiaoyan
AU  - Zhang X
FAU - Yan, Huimin
AU  - Yan H
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20120305
PL  - United States
TA  - Antimicrob Agents Chemother
JT  - Antimicrobial agents and chemotherapy
JID - 0315061
RN  - 0 (Anti-Infective Agents)
RN  - 0 (Benzalkonium Compounds)
RN  - 0 (Biomarkers)
RN  - 0 (Chemokine CCL2)
RN  - 0 (Interleukin-6)
RN  - 0 (Organophosphonates)
RN  - 0 (P-Selectin)
RN  - 126880-86-2 (L-Selectin)
RN  - 26027-38-3 (Nonoxynol)
RN  - 368GB5141J (Sodium Dodecyl Sulfate)
RN  - 99YXE507IL (Tenofovir)
RN  - JAC85A2161 (Adenine)
RN  - K679OBS311 (Carboxymethylcellulose Sodium)
SB  - IM
MH  - Adenine/adverse effects/analogs & derivatives
MH  - Animals
MH  - Anti-Infective Agents/*adverse effects
MH  - Benzalkonium Compounds/adverse effects
MH  - Biomarkers/*metabolism
MH  - Carboxymethylcellulose Sodium/adverse effects
MH  - Cervix Uteri/drug effects/metabolism
MH  - Chemokine CCL2
MH  - Female
MH  - HIV Infections/drug therapy
MH  - Inflammation/*chemically induced/*metabolism
MH  - Interleukin-6/metabolism
MH  - L-Selectin/*metabolism
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Mucous Membrane/drug effects
MH  - Nonoxynol/adverse effects/therapeutic use
MH  - Organophosphonates/adverse effects
MH  - P-Selectin/*metabolism
MH  - Sodium Dodecyl Sulfate/adverse effects
MH  - Tenofovir
PMC - PMC3370781
EDAT- 2012/03/07 06:00
MHDA- 2012/09/14 06:00
PMCR- 2012/12/01
CRDT- 2012/03/07 06:00
PHST- 2012/03/07 06:00 [entrez]
PHST- 2012/03/07 06:00 [pubmed]
PHST- 2012/09/14 06:00 [medline]
PHST- 2012/12/01 00:00 [pmc-release]
AID - AAC.05950-11 [pii]
AID - 05950-11 [pii]
AID - 10.1128/AAC.05950-11 [doi]
PST - ppublish
SO  - Antimicrob Agents Chemother. 2012 Jun;56(6):3121-32. doi: 10.1128/AAC.05950-11. 
      Epub 2012 Mar 5.