PMID- 22392134
OWN - NLM
STAT- MEDLINE
DCOM- 20121204
LR  - 20211021
IS  - 1432-1955 (Electronic)
IS  - 0932-0113 (Linking)
VI  - 111
IP  - 2
DP  - 2012 Aug
TI  - Cloning and characterization of Plasmodium vivax thioredoxin peroxidase-1.
PG  - 525-9
LID - 10.1007/s00436-012-2864-3 [doi]
AB  - Reactive oxygen species produced from hemoglobin digestion and the host immune 
      system could have adverse effects on malaria parasites. To protect themselves, 
      malaria parasites are highly dependent on the antioxidant enzymes, including 
      superoxide dismutases and thioredoxin-dependent peroxidases. To date, several 
      thioredoxin peroxidases (TPx) have been characterized in Plasmodium falciparum, 
      but the TPx in Plasmodium vivax has not yet been characterized. The complete 
      sequence of gene coding for thioredoxin peroxidase-1 of P. vivax (PvTPx-1) was 
      amplified by PCR and cloned. Using the recombinant PvTPx-1 (rPvTPx-1), polyclonal 
      antibody was produced in mice for immunolocalization of the enzyme in the 
      parasite. The antioxidant activity of rPvTPx-1 was evaluated by mixed-function 
      oxidation assay. PvTPx-1 has two conserved cysteine residues in the amino acid 
      sequence at the positions 50 and 170 which formed a dimer under a non-reducing 
      condition. Using a thiol mixed-function oxidation assay, the antioxidant activity 
      of rPvTPx-1 was revealed. Indirect immunofluorescence microscopy with the 
      specific antibody indicated that PvTPx-1 was expressed in the cytoplasm of the 
      erythrocytic stage of the parasite in a dots-like pattern. The results suggest 
      that P. vivax uses TPx-1 to reduce and detoxify hydrogen peroxides in order to 
      maintain their redox homeostasis and proliferation in the host body.
FAU - Hakimi, Hassan
AU  - Hakimi H
AD  - National Research Center for Protozoan Diseases, Obihiro University of 
      Agriculture and Veterinary Medicine, Inada-cho, Obihiro, Hokkaido 080-8555, 
      Japan.
FAU - Asada, Masahito
AU  - Asada M
FAU - Angeles, Jose Ma M
AU  - Angeles JM
FAU - Inoue, Noboru
AU  - Inoue N
FAU - Kawazu, Shin-Ichiro
AU  - Kawazu S
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20120306
PL  - Germany
TA  - Parasitol Res
JT  - Parasitology research
JID - 8703571
RN  - 0 (Antioxidants)
RN  - EC 1.11.1.15 (Peroxiredoxins)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Antioxidants
MH  - Cloning, Molecular
MH  - Fluorescent Antibody Technique, Indirect
MH  - Gene Expression Regulation, Enzymologic/*physiology
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Molecular Sequence Data
MH  - Peroxiredoxins/genetics/*metabolism
MH  - Plasmodium vivax/*enzymology
MH  - Polymerase Chain Reaction
EDAT- 2012/03/07 06:00
MHDA- 2012/12/10 06:00
CRDT- 2012/03/07 06:00
PHST- 2011/12/01 00:00 [received]
PHST- 2012/02/10 00:00 [accepted]
PHST- 2012/03/07 06:00 [entrez]
PHST- 2012/03/07 06:00 [pubmed]
PHST- 2012/12/10 06:00 [medline]
AID - 10.1007/s00436-012-2864-3 [doi]
PST - ppublish
SO  - Parasitol Res. 2012 Aug;111(2):525-9. doi: 10.1007/s00436-012-2864-3. Epub 2012 
      Mar 6.