PMID- 22393694
OWN - NLM
STAT- MEDLINE
DCOM- 20130114
LR  - 20161125
IS  - 1000-8713 (Print)
IS  - 1000-8713 (Linking)
VI  - 29
IP  - 11
DP  - 2011 Nov
TI  - [Determination of aristolochic acids A and B in Chinese herbals and traditional 
      Chinese patent medicines using ultra high performance liquid 
      chromatography-triple quadrupole mass spectrometry].
PG  - 1076-81
AB  - A method for the simultaneous determination of aristolochic acids A and B in some 
      Chinese herbals and traditional Chinese patent medicines by ultra high 
      performance liquid chromatography-triple quadrupole mass spectrometry 
      (UHPLC-MS/MS) was developed. Fourteen samples, including Radix Bupleuri, Radix 
      Glycyrrhizae, Radix Platycodonis, Longdanxieganwan, Xiaopangwan, Slimming Tea, 
      etc., were extracted with methanol-water (70: 30, v/v) and purified with Oasis 
      MAX solid-phase extraction cartridges, then analyzed on an Eclipse RP HD C18 
      column (150 mm x 2.1 mm, 1.8 microm) using 5 mmol/L ammonium acetate solution (pH 
      7.5)-acetonitrile (75: 25, v/v) as the mobile phase. The mass spectrometric 
      acquisition was carried out by means of electrospray ionization in positive mode 
      (ESI+) with multiple reaction monitoring (MRM) method. The good linearities (r2 > 
      0.995) were achieved within the ranges of 0.5 - 200 microg/L and 1 - 200 microg/L 
      for aristolochic acids A and B, respectively. The limits of detection (LODs) were 
      5 microg/kg for aristolochic A and 7.5 microg/kg for aristolochic B, and the 
      limits of quantification (LOQs) were 12.5 microg/kg and 25 microg/kg, 
      respectively. The recoveries of aristolochic acids A and B at the spiked levels 
      of 100 microg/kg and 500 microg/kg ranged from 60.3% to 96.4% with the relative 
      standard deviations (RSDs) not more than 10.2%. The results demonstrated that the 
      proposed method is efficient, sensitive, reproducible, reliable and suitable for 
      the trace determination of aristolochic acids A and B in Chinese herbals and 
      traditional Chinese patent medicines.
FAU - Liu, Ying
AU  - Liu Y
AD  - Inspection and Quarantine Technical Center of Beijing Entry-Exit Inspection and 
      Quarantine Bureau, Beijing 100026, China.
FAU - Han, Shen
AU  - Han S
FAU - Feng, Qian
AU  - Feng Q
FAU - Wang, Jinhua
AU  - Wang J
LA  - chi
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Se Pu
JT  - Se pu = Chinese journal of chromatography
JID - 9424804
RN  - 0 (Aristolochic Acids)
RN  - 0 (Drugs, Chinese Herbal)
RN  - 0 (aristolochic acid B)
RN  - 94218WFP5T (aristolochic acid I)
SB  - IM
MH  - Aristolochic Acids/*analysis
MH  - Chromatography, High Pressure Liquid/*methods
MH  - Drugs, Chinese Herbal/*chemistry
MH  - Spectrometry, Mass, Electrospray Ionization/methods
MH  - Tandem Mass Spectrometry/*methods
EDAT- 2012/03/08 06:00
MHDA- 2013/01/15 06:00
CRDT- 2012/03/08 06:00
PHST- 2012/03/08 06:00 [entrez]
PHST- 2012/03/08 06:00 [pubmed]
PHST- 2013/01/15 06:00 [medline]
PST - ppublish
SO  - Se Pu. 2011 Nov;29(11):1076-81.