PMID- 22393700
OWN - NLM
STAT- MEDLINE
DCOM- 20130114
LR  - 20161018
IS  - 1000-8713 (Print)
IS  - 1000-8713 (Linking)
VI  - 29
IP  - 11
DP  - 2011 Nov
TI  - [Determination of 5-hydroxytryptamine in plasma by nanofiber solid phase 
      extraction-high performance liquid chromatography].
PG  - 1107-11
AB  - A novel packed-nanofiber solid phase extraction coupled with high performance 
      liquid chromatography-electrochemical detection (HPLC-ECD) method was established 
      for the determination of 5-hydroxytryptamine (5-HT) in plasma. A 10% (v/v) HClO4 
      solution was used to precipitate the protein in plasma samples. After 
      homogenizing for 1 min and centrifuging for 10 min at 12 000 r/min, the 
      supernatant was adjusted to pH 8.5 with 0.1 mol/L sodium tetraphenylborate and 
      then derivatized with o-phthalaldehyde (OPA) solution at 30 degrees C for 4 min. 
      The solution was then purified and preconcentrated by the packed-nanofiber solid 
      phase extraction column, in which methanol was used as the eluent. The analyte 
      was analyzed by HPLC-ECD. The chromatographic separation was achieved on a 
      Shimadzu C18 column with pH 5. 4 0. 05 mol/L phosphate buffer solution 
      (containing 0.25 mmol/L ethylene diamine tetraacetic acid)-methanol (60:40, v/v) 
      as mobile phase. The linear range was 5 -500 microg/L with the correlation 
      coefficient (r2) of 0.9996. The limit of detection (S/N = 3) was 1 microg/L. And 
      the spiked recoveries were in the range of 95.6% - 101.4% with the relative 
      standard deviations (RSDs) of intra-day and inter-day assays below 5% (n = 3). On 
      the basis of the advantages of simplicity, high sensitivity and good 
      reproducibility, this method can be used for the determination of 5-HT in human 
      plasma.
FAU - Zhou, Xiaoling
AU  - Zhou X
AD  - Key Laboratory of Child Development and Learning Science (Ministry of Education), 
      Southeast University, Nanjing 210096, China.
FAU - Wang, Yu
AU  - Wang Y
FAU - Chen, Liqin
AU  - Chen L
FAU - Kang, Xuejun
AU  - Kang X
LA  - chi
PT  - English Abstract
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Se Pu
JT  - Se pu = Chinese journal of chromatography
JID - 9424804
RN  - 333DO1RDJY (Serotonin)
SB  - IM
MH  - Chromatography, High Pressure Liquid/*methods
MH  - Electrochemical Techniques/methods
MH  - Humans
MH  - *Nanofibers
MH  - Sensitivity and Specificity
MH  - Serotonin/*blood
MH  - Solid Phase Extraction/*methods
EDAT- 2012/03/08 06:00
MHDA- 2013/01/15 06:00
CRDT- 2012/03/08 06:00
PHST- 2012/03/08 06:00 [entrez]
PHST- 2012/03/08 06:00 [pubmed]
PHST- 2013/01/15 06:00 [medline]
PST - ppublish
SO  - Se Pu. 2011 Nov;29(11):1107-11.