PMID- 22393704
OWN - NLM
STAT- MEDLINE
DCOM- 20130114
LR  - 20161018
IS  - 1000-8713 (Print)
IS  - 1000-8713 (Linking)
VI  - 29
IP  - 11
DP  - 2011 Nov
TI  - [Determination of penicillin intermediate and three penicillins in milk by high 
      performance capillary electrophoresis].
PG  - 1128-32
AB  - A high performance capillary electrophoresis (HPCE) method was developed for the 
      simultaneous determination of penicillin intermediate and penicillins in milk, 
      including 6-amino-penicillanic acid (6-APA), penicillin G (PEN), ampicillin (AMP) 
      and amoxicillin (AMO). The main parameters including the ion concentration and pH 
      value of running buffer, separation voltage and column temperature were optimized 
      systematically by orthogonal test. The four penicillins (PENs) were baseline 
      separated within 4.5 min with the running buffer of 40 mmol/L potassium 
      dihydrogen phosphate-20 mmol/L borax solution (pH 7.8), separation voltage of 28 
      kV and column temperature of 30 degrees C. The calibration curves showed good 
      linearity in the range of 1.56 - 100 mg/L, and the correlation coefficients (r2) 
      were between 0.9979 and 0.9998. The average recoveries at three spiked levels 
      were in the range of 84.91% - 96.72% with acceptable relative standard deviations 
      (RSDs) of 1.11% - 9.11%. The method is simple, fast, accurate and suitable for 
      the determination of penicillins in real samples.
FAU - Tian, Chunqiu
AU  - Tian C
AD  - College of Tea & Food Science and Technology, Anhui Agricultural University, 
      Hefei 230036, China.
FAU - Tan, Huarong
AU  - Tan H
FAU - Gao, Liping
AU  - Gao L
FAU - Shen, Huqin
AU  - Shen H
FAU - Qi, Kezong
AU  - Qi K
LA  - chi
PT  - English Abstract
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Se Pu
JT  - Se pu = Chinese journal of chromatography
JID - 9424804
RN  - 7C782967RD (Ampicillin)
RN  - 804826J2HU (Amoxicillin)
RN  - 87-53-6 (Penicillanic Acid)
RN  - Q42T66VG0C (Penicillin G)
RN  - QR0C4R7XVN (aminopenicillanic acid)
SB  - IM
MH  - Amoxicillin/analysis/metabolism
MH  - Ampicillin/*analysis/metabolism
MH  - Animals
MH  - Cattle
MH  - Drug Residues/analysis
MH  - Electrophoresis, Capillary/*methods
MH  - Food Contamination/analysis
MH  - Milk/*chemistry
MH  - Penicillanic Acid/*analogs & derivatives/analysis
MH  - Penicillin G/*analysis/metabolism
EDAT- 2012/03/08 06:00
MHDA- 2013/01/15 06:00
CRDT- 2012/03/08 06:00
PHST- 2012/03/08 06:00 [entrez]
PHST- 2012/03/08 06:00 [pubmed]
PHST- 2013/01/15 06:00 [medline]
PST - ppublish
SO  - Se Pu. 2011 Nov;29(11):1128-32.