PMID- 22393722
OWN - NLM
STAT- MEDLINE
DCOM- 20130221
LR  - 20181201
IS  - 1000-3061 (Print)
IS  - 1000-3061 (Linking)
VI  - 27
IP  - 11
DP  - 2011 Nov
TI  - [Cloning, expression and characterization of gene encoding human stem cell growth 
      factor-alpha and its synergetic effect with rhGM-CSF on proliferation of human 
      umbilical cord mesenchymal stem cells].
PG  - 1667-76
AB  - To investigate the effect of hSCGF-alpha on human Umbilical Cord Mesenchymal Stem 
      Cells (hUCMSCs), we obtained hSCGF-alpha using genetic engineering, hSCGF-alpha 
      gene was amplified from hUCMSCs cDNA using two-step PCR and was inserted into 
      pET-28a(+) plasmid vector. Induced by IPTG at 20 degrees Celsius for 24 h, the 
      fusion protein expressed in E. coli BL21 (DE3) was mainly existing in soluble 
      form. The recombinant hSCGF-a was purified using NI-NTA affinity chromatography 
      and the purity was up to 90%. The colony forming test revealed that combined use 
      hSCGF-alpha and rmGM-CSF (recombinant murine GM-colony stimulating factor, 
      rmGM-CSF) had granulocyte/macrophage (GM) promoting effects on murine bone marrow 
      GM progenitor. In addition, the results indicated that hSCGF-alpha and rhGM-CSF 
      had stimulatory effect on hUCMSCs and their synergetic effect was the strongest.
FAU - Peng, Xinlei
AU  - Peng X
AD  - Guangdong Provincial Key Laboratory of Bioengineering Medicine, Biomedicine 
      Research and Development Centre of Jinan University, Guangzhou 510632, China.
FAU - Ma, Yanyan
AU  - Ma Y
FAU - Rong, Jing
AU  - Rong J
FAU - Zhao, Zhenling
AU  - Zhao Z
FAU - Han, Bo
AU  - Han B
FAU - Chen, Wei
AU  - Chen W
FAU - Xiang, Yangfei
AU  - Xiang Y
FAU - Liu, Qiuying
AU  - Liu Q
FAU - Wang, Yifei
AU  - Wang Y
FAU - Ren, Zhe
AU  - Ren Z
FAU - Zhou, Xiangrong
AU  - Zhou X
FAU - Chen, Haijia
AU  - Chen H
LA  - chi
PT  - Journal Article
PL  - China
TA  - Sheng Wu Gong Cheng Xue Bao
JT  - Sheng wu gong cheng xue bao = Chinese journal of biotechnology
JID - 9426463
RN  - 0 (Recombinant Fusion Proteins)
RN  - 0 (Stem Cell Factor)
RN  - 83869-56-1 (Granulocyte-Macrophage Colony-Stimulating Factor)
SB  - IM
MH  - Cell Proliferation/*drug effects
MH  - Cells, Cultured
MH  - Cloning, Molecular
MH  - Drug Synergism
MH  - Escherichia coli/genetics/metabolism
MH  - Granulocyte-Macrophage Colony-Stimulating Factor/*pharmacology
MH  - Humans
MH  - Mesenchymal Stem Cells/cytology/*metabolism
MH  - Recombinant Fusion Proteins/biosynthesis/genetics/pharmacology
MH  - Stem Cell Factor/*biosynthesis/genetics
MH  - Umbilical Cord/*cytology
EDAT- 2012/03/08 06:00
MHDA- 2013/02/22 06:00
CRDT- 2012/03/08 06:00
PHST- 2012/03/08 06:00 [entrez]
PHST- 2012/03/08 06:00 [pubmed]
PHST- 2013/02/22 06:00 [medline]
PST - ppublish
SO  - Sheng Wu Gong Cheng Xue Bao. 2011 Nov;27(11):1667-76.