PMID- 22394078
OWN - NLM
STAT- MEDLINE
DCOM- 20120827
LR  - 20150708
IS  - 1460-9568 (Electronic)
IS  - 0953-816X (Linking)
VI  - 35
IP  - 8
DP  - 2012 Apr
TI  - Lentiviral-mediated gene delivery reveals distinct roles of nucleus accumbens 
      dopamine D2 and D3 receptors in novelty- and light-induced locomotor activity.
PG  - 1344-53
LID - 10.1111/j.1460-9568.2012.08028.x [doi]
AB  - The importance of the dopaminergic system for proper brain activity is 
      demonstrated by findings that alterations in this system lead to severe 
      disabilities, including motor impairment observed in various neurological and 
      psychiatric disorders. Although the roles of specific dopamine receptors in 
      behaviour have been extensively investigated using pharmacological agents and 
      knockout mice, non-specificity of ligands and compensatory molecular adaptations 
      in mutated animals restrict the interpretation of the results. To overcome these 
      limitations and further explore the role of the dopamine D2 and D3 receptors (D2R 
      and D3R) in rats, we used lentivirus-mediated gene knockdown and overexpression 
      to specifically manipulate expression levels of these genes in the rat nucleus 
      accumbens (NAcc), a brain area important for spontaneous and induced locomotor 
      responses. Lentiviruses, inducing expression of rat D2R or D3R, or efficient 
      knockdown of either receptor by small hairpin (sh)RNAs, were stereotaxically 
      injected into the NAcc. While knockdown of either receptor significantly reduced 
      spontaneous locomotor activity in a novel but not in a habituated environment, 
      D2R and D3R appeared to contribute in opposite ways to light-induced locomotor 
      activity. D2R knockdown increased while D3R knockdown decreased locomotor 
      activity in this test. Altogether, our findings suggest that D2R and D3R, 
      expressed in the NAcc, have both shared and non-overlapping roles in transduction 
      of alerting signals elicited by potentially important sensory and environmental 
      cues.
CI  - (c) 2012 The Authors. European Journal of Neuroscience (c) 2012 Federation of 
      European Neuroscience Societies and Blackwell Publishing Ltd.
FAU - Fernandes, Alinda R
AU  - Fernandes AR
AD  - MRC-SGDP-Centre, Institute of Psychiatry, King's College London, De Crespigny 
      Park, PO80, London SE5 8AF, UK.
FAU - Easton, Alanna C
AU  - Easton AC
FAU - De Souza Silva, Maria A
AU  - De Souza Silva MA
FAU - Schumann, Gunter
AU  - Schumann G
FAU - Muller, Christian P
AU  - Muller CP
FAU - Desrivieres, Sylvane
AU  - Desrivieres S
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20120307
PL  - France
TA  - Eur J Neurosci
JT  - The European journal of neuroscience
JID - 8918110
RN  - 0 (Receptors, Dopamine D2)
RN  - 0 (Receptors, Dopamine D3)
RN  - 147336-22-9 (Green Fluorescent Proteins)
SB  - IM
MH  - Animals
MH  - Cell Line, Transformed
MH  - Exploratory Behavior/*physiology
MH  - Gene Expression Regulation/genetics
MH  - Gene Transfer Techniques
MH  - Genetic Vectors/physiology
MH  - Green Fluorescent Proteins/genetics
MH  - Humans
MH  - Lentivirus/genetics
MH  - Light
MH  - Male
MH  - Maze Learning/physiology
MH  - Motor Activity/*physiology
MH  - Nucleus Accumbens/*metabolism
MH  - Rats
MH  - Receptors, Dopamine D2/genetics/*metabolism
MH  - Receptors, Dopamine D3/genetics/*metabolism
MH  - Transfection
EDAT- 2012/03/08 06:00
MHDA- 2012/08/28 06:00
CRDT- 2012/03/08 06:00
PHST- 2012/03/08 06:00 [entrez]
PHST- 2012/03/08 06:00 [pubmed]
PHST- 2012/08/28 06:00 [medline]
AID - 10.1111/j.1460-9568.2012.08028.x [doi]
PST - ppublish
SO  - Eur J Neurosci. 2012 Apr;35(8):1344-53. doi: 10.1111/j.1460-9568.2012.08028.x. 
      Epub 2012 Mar 7.