PMID- 22397583
OWN - NLM
STAT- MEDLINE
DCOM- 20120807
LR  - 20211021
IS  - 1520-5827 (Electronic)
IS  - 0743-7463 (Print)
IS  - 0743-7463 (Linking)
VI  - 28
IP  - 13
DP  - 2012 Apr 3
TI  - Determination of peptide-surface adsorption free energy for material surfaces not 
      conducive to SPR or QCM using AFM.
PG  - 5687-94
LID - 10.1021/la300315r [doi]
AB  - The interactions between peptides and proteins with material surfaces are of 
      primary importance in many areas of biotechnology. While surface plasmon 
      resonance spectroscopy (SPR) and quartz crystal microbalance (QCM) methods have 
      proven to be very useful in measuring fundamental properties characterizing 
      adsorption behavior, such as the free energy of adsorption for peptide-surface 
      interactions, these methods are largely restricted to use for materials that can 
      readily form nanoscale-thick films over the respective sensor surfaces. Many 
      materials including most polymers, ceramics, and inorganic glasses, however, are 
      not readily suitable for use with SPR or QCM methods. To overcome these 
      limitations, we recently showed that desorption forces (F(des)) obtained using a 
      standardized AFM method linearly correlate to standard-state adsorption free 
      energy values (DeltaG degrees (ads)) measured from SPR in phosphate buffered saline (PBS: 
      phosphate buffered 140 mM NaCl, pH 7.4). This approach thus provides a means to 
      determine DeltaG degrees (ads) for peptide adsorption using AFM that can be applied to any 
      flat material surface. In this present study, we investigated the F(des)-DeltaG degrees (ads) 
      correlation between AFM and SPR data in PBS for a much broader range of systems 
      including eight different types of peptides on a set of eight different 
      alkanethiol self-assembled monolayer (SAM) surfaces. The resulting correlation 
      was then used to estimate DeltaG degrees (ads) from F(des) determined by AFM for selected 
      bulk polymer and glass/ceramic materials such as poly(methyl methacrylate) 
      (PMMA), high-density polyethylene (HDPE), fused silica glass, and a quartz (100) 
      surface. The results of these studies support our previous findings regarding the 
      strong correlation between F(des) measured by AFM and DeltaG degrees (ads) determined by SPR, 
      and provides a means to estimate DeltaG degrees (ads) for peptide adsorption on 
      macroscopically thick samples of materials that are not conducive for use with 
      SPR or QCM.
FAU - Thyparambil, Aby A
AU  - Thyparambil AA
AD  - Department of Bioengineering, Clemson University, Clemson, South Carolina 29634, 
      United States.
FAU - Wei, Yang
AU  - Wei Y
FAU - Latour, Robert A
AU  - Latour RA
LA  - eng
GR  - P41 EB002027/EB/NIBIB NIH HHS/United States
GR  - P41 EB002027-28/EB/NIBIB NIH HHS/United States
GR  - EB002027/EB/NIBIB NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, U.S. Gov't, Non-P.H.S.
DEP - 20120320
PL  - United States
TA  - Langmuir
JT  - Langmuir : the ACS journal of surfaces and colloids
JID - 9882736
RN  - 0 (Buffers)
RN  - 0 (Peptides)
SB  - IM
MH  - Adsorption
MH  - Buffers
MH  - Hydrogen-Ion Concentration
MH  - Microscopy, Atomic Force
MH  - Peptides/*chemistry
MH  - *Quartz Crystal Microbalance Techniques
MH  - *Surface Plasmon Resonance
MH  - Surface Properties
MH  - Thermodynamics
PMC - PMC3319500
MID - NIHMS362747
EDAT- 2012/03/09 06:00
MHDA- 2012/08/08 06:00
PMCR- 2013/04/03
CRDT- 2012/03/09 06:00
PHST- 2012/03/09 06:00 [entrez]
PHST- 2012/03/09 06:00 [pubmed]
PHST- 2012/08/08 06:00 [medline]
PHST- 2013/04/03 00:00 [pmc-release]
AID - 10.1021/la300315r [doi]
PST - ppublish
SO  - Langmuir. 2012 Apr 3;28(13):5687-94. doi: 10.1021/la300315r. Epub 2012 Mar 20.