PMID- 22399175
OWN - NLM
STAT- MEDLINE
DCOM- 20130107
LR  - 20201209
IS  - 0374-9096 (Print)
IS  - 0374-9096 (Linking)
VI  - 46
IP  - 1
DP  - 2012 Jan
TI  - [Investigation of Microsporidia prevalence by different staining methods in cases 
      of diarrhea].
PG  - 85-92
AB  - Microsporidia, depending on their different species, generally lead to 
      self-limited, sporadic and mild infections such as diarrhea, corneal ulcer and 
      myositis. They are considered as opportunistic pathogens in HIV-positive patients 
      however in recent years Microsporidia have been detected also in immunocompetent 
      individuals as a cause of diarrhea. Diagnosis of Microsporidia depends on the 
      detection of spores or different developmental stages of protozoon in stool, 
      urine, sinus aspirates, nasal discharge, bronchoalveolar lavage or tissue 
      biopsies. Diagnosis of Microsporidia infections is usually achieved by the use of 
      different staining methods, serological tests, polymerase chain reaction, and 
      electron microscopic methods. The aims of this study were to detect the incidence 
      of microsporidia in patients with diarrhea by using three different staining 
      methods and to compare the performance of these methods. A total of 225 stool 
      samples from diarrheal patients (84 were children, 141 were adults; 103 were 
      female, 122 were male) admitted to Gazi University Medical Faculty Hospital 
      between March-June 2009, have been evaluated in the laboratory of Medical 
      Microbiology Department. Stool samples were examined in terms of the presence of 
      Microsporidia spores by Weber's modified trichrom staining (MTS), calcofluor (CF) 
      and acridine orange (AO) staining methods. Microsporidia positivity rate was 9.8% 
      (22/225) in the diarrheal patients, the rate being 9.5% (8/84) in children and 
      9.9% (14/141) in adults. There was no statistically significant difference 
      between age and gender groups (p> 0.05) regarding Microsporidia detection. When 
      MTS was considered as the reference method, sensitivity, specifity and 
      consistency of AO staining were estimated as 100%, 91.6% and 92%, respectively, 
      while those rates for CF staining were 95.4%, 99.5% and 99%, respectively. There 
      was very strong and significant correlation (r= 0.950, p< 0.001) between CF 
      staining and MTS, while there was strong and significant (r= 0.719, p< 0.001) 
      correlation between AO staining and MTS. Although AO staining is rapid and 
      convenient, the positive predictive value was measured very low (56.4%) and the 
      interpretation of stained slides was very difficult since background of the 
      slides was stained orange and there were a lot of dye artefacts. In conclusion, 
      screening Microsporidia in all diarrheal stool samples is of diagnostic value. To 
      increase sensitivity and reliability in the detection of Microsporidia spores in 
      diarrheal samples, initial application of calcofluor staining should be followed 
      by the confirmatory MTS method.
FAU - Turk, Songul
AU  - Turk S
AD  - Gazi University Institute of Health Sciences, Ankara, Turkey.
FAU - Dogruman Al, Funda
AU  - Dogruman Al F
FAU - Karaman, Ulku
AU  - Karaman U
FAU - Kustimur, Semra
AU  - Kustimur S
LA  - tur
PT  - Journal Article
TT  - Ishalli Olgularda Microsporidia Sikliginin Farkli Boyama Yontemleriyle 
      Arastirilmasi.
PL  - Turkey
TA  - Mikrobiyol Bul
JT  - Mikrobiyoloji bulteni
JID - 7503830
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Child
MH  - Child, Preschool
MH  - Diarrhea/epidemiology/*microbiology
MH  - Feces/microbiology
MH  - Female
MH  - Humans
MH  - Incidence
MH  - Infant
MH  - Male
MH  - Microsporidia/*isolation & purification
MH  - Microsporidiosis/epidemiology/*microbiology
MH  - Spores, Fungal/classification/isolation & purification
MH  - Turkey/epidemiology
MH  - Young Adult
EDAT- 2012/03/09 06:00
MHDA- 2013/01/08 06:00
CRDT- 2012/03/09 06:00
PHST- 2012/03/09 06:00 [entrez]
PHST- 2012/03/09 06:00 [pubmed]
PHST- 2013/01/08 06:00 [medline]
PST - ppublish
SO  - Mikrobiyol Bul. 2012 Jan;46(1):85-92.